Rathnayake Hasanga, De Zoysa Manikkuwadura Hasara Nethmini, Hewawasam Ruwani Punyakanthi, Wijayaratne Weerasinghe Mudiyanselage Dilip Gaya Bandara
Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Matara, Sri Lanka.
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Ruhuna, Matara, Sri Lanka.
Scientifica (Cairo). 2020 Jul 14;2020:8239053. doi: 10.1155/2020/8239053. eCollection 2020.
Methicillin-resistant (MRSA) is a major cause of hospital- and community-acquired infections worldwide. Therefore, this study was conducted to explore the antibacterial activity of the two medicinal plants and against strains of MRSA which were isolated from patients with skin and soft tissue infections. Hexane, ethanol, and water extracts of (whole plant) and (roots) were prepared. Clinical isolates of MRSA strains ( = 20) were used for the study. Bacterial susceptibility was tested using a disc diffusion assay. Minimum inhibitory concentration (MIC) was determined by a broth microdilution method. Vancomycin was used as the positive control. Hexane, ethanol, and water extracts of showed inhibitory zones against MRSA. Except for water extract, both hexane and ethanol extracts of showed inhibitory zones. MIC ranges of hexane, ethanol, and water extracts in were 0.012-0.32 mg/mL, 0.019-2.4 mg/mL, and 0.019-0.48 mg/mL, respectively. Respective MIC ranges of hexane and ethanol extracts of were 0.003-0.032 mg/mL and 0.019-2.4 mg/mL. The hexane extract of inhibited 55% of the selected MRSA strains at a relatively low MIC value of 0.012 mg/mL. The hexane extract of both plants demonstrated inhibition of 75% of MRSA strains at a MIC value of 0.064 mg/mL. Ethanol extract of and respectively, inhibited 70% and 45% of MRSA strains at the MIC of 0.096 mg/mL, whereas water extract of inhibited 80% of MRSA strains at the same MIC. Both and were equally effective against MRSA at a MIC of 0.064 mg/mL. But was more effective since the hexane extract inhibited more than 50% of MRSA strains at significantly a lower MIC value of 0.012 mg/mL. Fractionation, purification, and identification of active compounds will warrant further evaluation of the therapeutic potential of both plant extracts.
耐甲氧西林金黄色葡萄球菌（MRSA）是全球医院获得性感染和社区获得性感染的主要原因。因此，本研究旨在探讨两种药用植物对从皮肤和软组织感染患者中分离出的MRSA菌株的抗菌活性。制备了[植物名称1]（全株）和[植物名称2]（根）的己烷、乙醇和水提取物。使用20株MRSA临床分离株进行研究。采用纸片扩散法检测细菌敏感性。通过肉汤微量稀释法测定最低抑菌浓度（MIC）。万古霉素用作阳性对照。[植物名称1]的己烷、乙醇和水提取物对MRSA显示出抑菌圈。除水提取物外，[植物名称1]的己烷和乙醇提取物均显示出抑菌圈。[植物名称1]中己烷、乙醇和水提取物的MIC范围分别为0.012 - 0.32mg/mL、0.019 - 2.4mg/mL和0.019 - 0.48mg/mL。[植物名称2]的己烷和乙醇提取物的MIC范围分别为0.003 - 0.032mg/mL和0.019 - 2.4mg/mL。[植物名称1]的己烷提取物在相对较低的MIC值0.012mg/mL时抑制了55%的所选MRSA菌株。两种植物的己烷提取物在MIC值为0.064mg/mL时均抑制了75%的MRSA菌株。[植物名称1]和[植物名称2]的乙醇提取物在MIC为0.096mg/mL时分别抑制了70%和45%的MRSA菌株，而[植物名称1]的水提取物在相同MIC时抑制了80%的MRSA菌株。在MIC为0.064mg/mL时，[植物名称1]和[植物名称2]对MRSA的效果相同。但[植物名称1]更有效，因为其己烷提取物在显著更低的MIC值0.012mg/mL时抑制了超过50%的MRSA菌株。活性化合物的分离、纯化和鉴定将保证对两种植物提取物的治疗潜力进行进一步评估。
