Pharmaceutical Technology Department, National Research Center, Dokki, Cairo, Egypt; Faculty of Pharmacy, October University for Modern Sciences and Arts, Egypt.
Textile Research Division, National Research Center, Dokki, Cairo, Egypt.
Carbohydr Polym. 2017 Feb 20;158:11-19. doi: 10.1016/j.carbpol.2016.11.054. Epub 2016 Nov 22.
Burn wounds environment favors the growth of micro-organisms causing delay in wound healing. The traditional treatment with antimicrobial creams offer inaccurate doses. In the present study, a dressing coated with silver sulfadiazine (SSD) loaded chitosan nanoparticles (CSNPs) for the controlled-release of SSD into burn wound to control bacterial growth was investigated. CSNPs were formulated with different concentrations of chitosan and CM-β-CD and loaded with SSD complexed in 1:1 molar ratio with CM-β-CD, CSNPs were assessed for their particle size, polydispersity index, morphology and association efficiency. The formula showing the best characteristics was selected for the preparation of SSD loaded CSNPs wound dressing through a padding process with/without the use of cross-linker. The dressing was characterized for its physical properties, in addition, FTIR, X-ray, SEM and in vitro release were used for characterization. The dressing was proven effective for the inhibition of the growth of Gram positive and Gram negative bacteria as well as candida on an infected wound.
烧伤创面的环境有利于微生物的生长，导致伤口愈合延迟。传统的抗菌乳膏治疗方法提供的剂量并不准确。在本研究中，研究了一种用银磺胺嘧啶（SSD）负载壳聚糖纳米粒子（CSNPs）涂层的敷料，用于将 SSD 控释到烧伤创面以控制细菌生长。用不同浓度的壳聚糖和 CM-β-CD 制备 CSNPs，并将 SSD 与 CM-β-CD 以 1:1 摩尔比络合装载到 CSNPs 中，评估 CSNPs 的粒径、多分散指数、形态和结合效率。选择表现出最佳特性的配方，通过垫衬工艺（有/无交联剂）制备负载 SSD 的 CSNPs 伤口敷料。对敷料的物理性质进行了表征，此外，还使用傅里叶变换红外光谱（FTIR）、X 射线、扫描电子显微镜（SEM）和体外释放进行了表征。结果表明，该敷料能有效抑制感染创面革兰氏阳性和革兰氏阴性细菌以及念珠菌的生长。
