NanoEngineering Group, Faculty of Mechanical Engineering, Technion - Israel Institute of Technology, 32000 Haifa, Israel.
NanoEngineering Group, Faculty of Mechanical Engineering, Technion - Israel Institute of Technology, 32000 Haifa, Israel.
Carbohydr Polym. 2017 Feb 20;158:68-76. doi: 10.1016/j.carbpol.2016.12.003. Epub 2016 Dec 5.
Effective encapsulation and protection of biotherapeutics using a bio-based carrier, preferably issued from renewable resources, remains a challenge. Herein, we demonstrate application of coaxial electrospinning to fabric starch-based core-sheath compound fibers as a bacterial cells' carrier. Starch-formate is employed as an encapsulation agent, while the fiber core is made of glycerol, serving as a cell suspension medium. SEM microscopy reveals a distinct core-sheath morphology of the starch-formate/glycerol (SFG) compound fibers with mean diameters of 4.13±1.05μm. Calorimetric and thermomechanical analyses and FTIR spectroscopy show a progressive interaction between the starch-formate and the glycerol with time, pronounced with temperature increase. SFG fibers with encapsulated Lactobacillus paracasei are proved stable with retained bacterial viability when stored at 4°C and room temperature for up to 21days. SFG fibers present a potential biotherapeutic products' encapsulation platform, guaranteeing the stability at refrigerated and ambient storage conditions, as determined in this study.
使用生物基载体（最好来自可再生资源）对生物疗法进行有效包封和保护仍然是一个挑战。在此，我们展示了同轴静电纺丝在制备以淀粉为基础的核-鞘复合纤维作为细菌细胞载体中的应用。淀粉甲酸盐被用作包封剂，而纤维芯由甘油制成，用作细胞悬浮介质。SEM 显微镜显示，淀粉甲酸盐/甘油（SFG）复合纤维具有明显的核-鞘形态，平均直径为 4.13±1.05μm。量热法和热机械分析以及 FTIR 光谱表明，淀粉甲酸盐和甘油之间存在渐进的相互作用，随着温度的升高而变得明显。用包封的副干酪乳杆菌的 SFG 纤维在 4°C 和室温下储存长达 21 天时表现出稳定的状态，并且保持细菌活力。SFG 纤维为生物治疗产品的包封平台提供了潜力，保证了在冷藏和环境储存条件下的稳定性，正如本研究所确定的。
