Pozo Francisco, Juste Javier, Vázquez-Morón Sonia, Aznar-López Carolina, Ibáñez Carlos, Garin Inazio, Aihartza Joxerra, Casas Inmaculada, Tenorio Antonio, Echevarría Juan Emilio
Virology Section, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Estación Biológica de Doñana, CSIC, Seville, Spain.
PLoS One. 2016 Dec 30;11(12):e0169153. doi: 10.1371/journal.pone.0169153. eCollection 2016.
A thorough search for bat herpesviruses was carried out in oropharyngeal samples taken from most of the bat species present in the Iberian Peninsula from the Vespertilionidae, Miniopteridae, Molossidae and Rhinolophidae families, in addition to a colony of captive fruit bats from the Pteropodidae family. By using two degenerate consensus PCR methods targeting two conserved genes, distinct and previously unrecognized bat-hosted herpesviruses were identified for the most of the tested species. All together a total of 42 potentially novel bat herpesviruses were partially characterized. Thirty-two of them were tentatively assigned to the Betaherpesvirinae subfamily while the remaining 10 were allocated into the Gammaherpesvirinae subfamily. Significant diversity was observed among the novel sequences when compared with type herpesvirus species of the ICTV-approved genera. The inferred phylogenetic relationships showed that most of the betaherpesviruses sequences fell into a well-supported unique monophyletic clade and support the recognition of a new betaherpesvirus genus. This clade is subdivided into three major clades, corresponding to the families of bats studied. This supports the hypothesis of a species-specific parallel evolution process between the potentially new betaherpesviruses and their bat hosts. Interestingly, two of the betaherpesviruses' sequences detected in rhinolophid bats clustered together apart from the rest, closely related to viruses that belong to the Roseolovirus genus. This suggests a putative third roseolo lineage. On the contrary, no phylogenetic structure was detected among several potentially novel bat-hosted gammaherpesviruses found in the study. Remarkably, all of the possible novel bat herpesviruses described in this study are linked to a unique bat species.
我们对伊比利亚半岛上大多数蝙蝠物种的口咽样本进行了全面的蝙蝠疱疹病毒搜索，这些蝙蝠物种来自蝙蝠科、鼠耳蝠科、犬吻蝠科和菊头蝠科，此外还包括一群圈养的狐蝠科果蝠。通过使用两种针对两个保守基因的简并一致性PCR方法，在大多数测试物种中鉴定出了不同的、以前未被识别的蝙蝠宿主疱疹病毒。总共对42种潜在的新型蝙蝠疱疹病毒进行了部分特征描述。其中32种暂时被归类为β疱疹病毒亚科，其余10种被归类为γ疱疹病毒亚科。与国际病毒分类委员会认可属的典型疱疹病毒物种相比，新序列中观察到了显著的多样性。推断的系统发育关系表明，大多数β疱疹病毒序列落入一个得到充分支持的独特单系分支，支持了一个新的β疱疹病毒属的认可。这个分支被细分为三个主要分支，对应于所研究的蝙蝠科。这支持了潜在的新β疱疹病毒与其蝙蝠宿主之间物种特异性平行进化过程的假设。有趣的是，在菊头蝠中检测到的两种β疱疹病毒序列聚集在一起，与属于玫瑰病毒属的病毒密切相关。这表明存在一个假定的第三个玫瑰病毒谱系。相反，在该研究中发现的几种潜在的新型蝙蝠宿主γ疱疹病毒之间未检测到系统发育结构。值得注意的是，本研究中描述的所有可能的新型蝙蝠疱疹病毒都与一种独特的蝙蝠物种相关。
