Mateus-Vargas Rafael H, Atanassova Viktoria, Reich Felix, Klein Günter
Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Food Microbiol. 2017 May;63:164-169. doi: 10.1016/j.fm.2016.11.013. Epub 2016 Nov 26.
The increasing number of antimicrobial resistant Enterobacteriaceae both in veterinary and human medicine, the dissemination of these bacteria in several environments and their possible repercussions on human health is causing concern. Game meat is usually seen as free of antimicrobial resistant bacteria. The objective of this study was to evaluate the current antimicrobial susceptibility status in generic Escherichia coli isolated from packed frozen game meat from a game handling establishment in Germany. A total of 229 E. coli isolates were obtained from cuts of red deer, roe deer and wild boar. The susceptibility to 12 antimicrobial agents was evaluated by a broth microdilution method according to ISO 20776-1:2006. Minimal Inhibitory Concentration (MIC) values were compared to breakpoints and cut-off values published by the EUCAST. Isolates showing MICs above the reference values were further studied for associated resistance determinants and phylogrouping by PCR. Overall, 16 E. coli isolates (7.0%) showed resistance (microbiological or clinical) to at least one antimicrobial agent tested. Clinical resistance was recorded to ampicillin (5/229) and chloramphenicol (4/229), whereas the MIC of 9 isolates exceeded the epidemiological cut-off value for doxycycline. One of the ampicillin-resistant isolates showed resistance to the β-lactam antibiotic derivatives tested, cephalosporines and aztreonam. Three of 9 non-wild-type isolates for doxycycline were positive for tet (B) genes. The ß-lactam-resistant isolate was found to harbour bla gene. These data show a low prevalence of resistant E. coli in packed game meat compared to studies on conventional meat. Although isolates obtained in this study may also be originating from the processing environment and not necessarily from animals, based on our results, it is important to monitor the development of antimicrobial resistance in game animals and products in order to identify future threats for the consumers.
兽医和人类医学中对抗菌药物耐药的肠杆菌科细菌数量不断增加，这些细菌在多种环境中的传播及其对人类健康可能产生的影响令人担忧。野味通常被视为不含抗菌药物耐药菌。本研究的目的是评估从德国一家野味处理机构的冷冻包装野味肉中分离出的普通大肠杆菌目前的抗菌药物敏感性状况。总共从马鹿、狍和野猪的肉块中获得了229株大肠杆菌分离株。根据ISO 20776-1:2006，采用肉汤微量稀释法评估了对12种抗菌药物的敏感性。将最低抑菌浓度（MIC）值与欧盟CAST公布的断点值和临界值进行比较。对MIC高于参考值的分离株进一步通过PCR研究相关耐药决定因素和系统发育分组。总体而言，16株大肠杆菌分离株（7.0%）对至少一种测试抗菌药物表现出耐药性（微生物学或临床耐药）。记录到对氨苄西林（5/229）和氯霉素（4/229）的临床耐药，而9株分离株的MIC超过了多西环素的流行病学临界值。一株氨苄西林耐药分离株对测试的β-内酰胺类抗生素衍生物、头孢菌素和氨曲南表现出耐药。9株多西环素非野生型分离株中有3株tet（B）基因呈阳性。发现β-内酰胺耐药分离株携带bla基因。与传统肉类研究相比，这些数据表明包装野味肉中耐药大肠杆菌的流行率较低。尽管本研究中获得的分离株也可能源自加工环境，不一定来自动物，但根据我们的结果，监测野味动物和产品中抗菌药物耐药性的发展对于识别未来对消费者的威胁很重要。
