不同品种的刺梨（Euterpe oleracea）的水醇提取物具有抗氧化潜力，并能保护人神经母细胞瘤细胞（SH-SY5Y）。
Hydroethanolic extracts from different genotypes of açaí (Euterpe oleracea) presented antioxidant potential and protected human neuron-like cells (SH-SY5Y).
机构信息
Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Prédio 43.212, Campus do Vale, Porto Alegre, RS CEP 91501-970, Brazil.
Embrapa Amazônia Oriental, Av. Dr. Enéas Pinheiro, s/n - Marco, Belém, PA CEP 66095-100, Brazil.
出版信息
Food Chem. 2017 May 1;222:94-104. doi: 10.1016/j.foodchem.2016.12.006. Epub 2016 Dec 7.
Fruit breeding programs have resulted in bioactive compounds increase and health effects. Thus, this study aimed to evaluate the antioxidant activity and neuroprotective effects of the hydroethanolic extracts from six açaí (Euterpe oleracea) genotypes using ABTS, deoxyribose, and glutathione oxidation assays, as well as, SH-SY5Y cells insulted with HO. L22P13 genotype showed the highest total content of anthocyanins, while L06P13 showed a high content of total carotenoids. However, the genotypes showed no difference in the antioxidant activity by ABTS and deoxyribose assays. The hydroethanolic extracts from different genotypes of açaí showed a protective effect (13-62%) on SH-SY5Y cells insulted by HO at a concentration of 50μg/mL by DCFH-DA assay. Except L04P16, no genotypes showed cytotoxicity in the SRB assay. These results indicate that açaí genotypes have antioxidant effect against reactive species generated in SH-SY5Y cells, suggesting a neuroprotective effect of the hydroethanolic extracts from these fruits.
水果培育计划导致生物活性化合物增加和健康影响。因此，本研究旨在使用 ABTS、脱氧核糖和谷胱甘肽氧化测定法评估六种巴西莓（Euterpe oleracea）基因型的水醇提取物的抗氧化活性和神经保护作用，以及用 HO 损伤 SH-SY5Y 细胞。L22P13 基因型的花色苷总含量最高，而 L06P13 则显示出较高的类胡萝卜素总含量。然而，不同基因型的巴西莓在 ABTS 和脱氧核糖测定法中的抗氧化活性没有差异。通过 DCFH-DA 测定法，不同基因型的巴西莓水醇提取物在浓度为 50μg/mL 时对 HO 损伤的 SH-SY5Y 细胞具有保护作用（13-62%）。除 L04P16 外，SRB 测定法中没有基因型显示细胞毒性。这些结果表明，巴西莓基因型对 SH-SY5Y 细胞中产生的活性物质具有抗氧化作用，表明这些水果的水醇提取物具有神经保护作用。
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