i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.
Departamento de Biomedicina, Unidade de Bioquímica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal.
Molecules. 2021 Apr 1;26(7):2011. doi: 10.3390/molecules26072011.
The fruit (açaí) is a promising source of polyphenols with health-promoting properties. To our knowledge, few studies have focused on the influence of açaí phytochemicals on angiogenesis, with a significant impact on cancer. This study aimed at investigating the phytochemical profile of a purple açaí hydroethanolic extract (AHE) obtained from a commercial dietary powder supplement by high-performance liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry, and evaluate its in vitro effects on distinct angiogenic steps during vessel growth and on oxidative markers in human microvascular endothelial cells (HMEC-1). The phenolic profile of AHE revealed the presence of significant levels of anthocyanins, mainly cyanidin-3--rutinoside, and other flavonoids with promising health effects. The in vitro studies demonstrated that AHE exerts antiangiogenic activity with no cytotoxic effect. The AHE was able to decrease HMEC-1 migration and invasion potential, as well as to inhibit the formation of capillary-like structures. Additionally, AHE increased antioxidant defenses by upregulating superoxide dismutase and catalase enzymatic activities, accompanied by a reduction in the production of reactive oxygen species. These data bring new insights into the potential application of angiogenic inhibitors present in AHE on the development of novel therapeutic approaches for angiogenesis-dependent diseases.
这种水果（阿萨伊）是一种具有促进健康特性的多酚的有前途的来源。据我们所知，很少有研究关注阿萨伊植物化学物质对血管生成的影响，而这对癌症有重大影响。本研究旨在通过高效液相色谱-二极管阵列检测和电喷雾电离质谱联用技术，对一种紫色阿萨伊水醇提取物（AHE）的植物化学特征进行研究，该提取物来自商业饮食粉补充剂，并评估其在体外对血管生长过程中不同血管生成步骤和人微血管内皮细胞（HMEC-1）中氧化标志物的影响。AHE 的酚类成分分析显示，存在大量的花色苷，主要是矢车菊素-3-芸香糖苷，以及其他具有潜在健康影响的类黄酮。体外研究表明，AHE 具有抗血管生成活性，无细胞毒性。AHE 能够降低 HMEC-1 的迁移和侵袭能力，并抑制毛细血管样结构的形成。此外，AHE 通过上调超氧化物歧化酶和过氧化氢酶的酶活性来增加抗氧化防御能力，同时减少活性氧的产生。这些数据为 AHE 中存在的血管生成抑制剂在开发依赖血管生成的疾病的新型治疗方法方面的潜在应用提供了新的见解。
