通过硫醇-烯或硫醇-炔偶联化学在多个点锚定探针提高DNA微阵列多重杂交性能。
Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry.
作者信息
Bañuls Maria-Jose, Jiménez-Meneses Pilar, Meyer Albert, Vasseur Jean-Jacques, Morvan François, Escorihuela Jorge, Puchades Rosa, Maquieira Ángel
机构信息
Interuniversitary Research Institute for Molecular Recognition and Technological Development (IDM), Chemistry Department, Universitat Politècnica de València , Camino de Vera s/n, 46022 Valencia, Spain.
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université de Montpellier , ENSCM, place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
出版信息
Bioconjug Chem. 2017 Feb 15;28(2):496-506. doi: 10.1021/acs.bioconjchem.6b00624. Epub 2017 Jan 20.
Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.
基于核酸微阵列的检测技术在可重复性、可靠性和分析灵敏度方面存在不足。在此，建立了一种基于硅基材料的探针附着模式的新策略。因此，通过将硫醇-烯或硫醇-炔点击化学反应与多硫醇化探针的多点附着相结合，杂交能力得到增强。通过特异性测定灵敏度高达20 pM的沙门氏菌PCR产物，证明了该方法的可行性和性能。
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