Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
Medical College, Shihezi University, Shihezi 832002, China.
J Diabetes Res. 2016;2016:5957404. doi: 10.1155/2016/5957404. Epub 2016 Dec 6.
We investigated the expression levels of both mRNA and protein as well as evaluating DNA methylation in the blood of type 2 diabetes mellitus (T2DM) Uyghur patients from Xinjiang. This study also evaluated whether gene expression had demonstrated any associations with clinical and biochemical indicators of T2DM. One hundred Uyghur subjects where divided into two groups, T2DM and nonimpaired glucose tolerance (NGT) groups. DNA methylation of was also analyzed by Spectrometry and methylation data of individual units were generated by the EpiTyper v1.0.5 software. The expression levels of and the protein expression levels were analyzed. Significant differences were observed in mRNA and protein levels when compared with the NGT group, while methylation rates of eight CpG units within the gene were higher in the T2DM group. Methylation of CpG sites was found to inversely correlate with expression of other markers. Results show that a correlation between mRNA, protein, and DNA methylation of gene exists among T2DM patients from Uyghur. protein and mRNA were downregulated and the DNA became hypermethylated, all of which may be involved in T2DM pathogenesis in this population.
我们研究了来自新疆的 2 型糖尿病（T2DM）维吾尔族患者血液中 mRNA 和蛋白质的表达水平，以及 DNA 甲基化情况。本研究还评估了基因表达是否与 T2DM 的临床和生化指标存在关联。
100 名维吾尔族受试者被分为 T2DM 组和糖耐量正常（NGT）组。采用质谱法分析 的 DNA 甲基化，通过 EpiTyper v1.0.5 软件生成单个单位的甲基化数据。分析 和蛋白的表达水平。与 NGT 组相比，mRNA 和蛋白水平存在显著差异，而 基因内 8 个 CpG 单位的甲基化率在 T2DM 组中更高。CpG 位点的甲基化与其他标志物的表达呈负相关。
结果表明，维吾尔族 T2DM 患者中 基因的 mRNA、蛋白和 DNA 甲基化之间存在相关性。 蛋白和 mRNA 下调，DNA 过度甲基化，这可能都与该人群的 T2DM 发病机制有关。
