Liu Xiaojing, Liu Lu, Wang Jie, Cui Huanxian, Zhao Guiping, Wen Jie
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Animal Science and Technology, College of Veterinary Medicine of Zhejiang A&F University, Hangzhou, China.
Front Physiol. 2021 Jul 6;12:682441. doi: 10.3389/fphys.2021.682441. eCollection 2021.
The glycogen content in muscle of livestock and poultry animals affects the homeostasis of their body, growth performance, and meat quality after slaughter. FOS-like 2, AP-1 transcription factor subunit (FOSL2) was identified as a candidate gene related to muscle glycogen (MG) content in chicken in our previous study, but the role of FOSL2 in the regulation of MG content remains to be elucidated. Differential gene expression analysis and weighted gene coexpression network analysis (WGCNA) were performed on differentially expressed genes (DEGs) in breast muscle tissues from the high-MG-content (HMG) group and low-MG-content (LMG) group of Jingxing yellow chickens. Analysis of the 1,171 DEGs (LMG vs. HMG) identified, besides , some additional genes related to MG metabolism pathway, namely , , , , and . Additionally, WGCNA revealed that , , , , , , , and other genes related to the classical glycogen metabolism in the same coexpressed module are associated with MG content. Also, besides finding that expression is negatively correlated with MG content, a possible interaction between and was predicted using the STRING (Search Tool for the Retrieval of Interacting Genes) database. Furthermore, we investigated the effects of lentiviral overexpression of FOSL2 on the regulation of the glycogen content , and the result indicated that FOSL2 decreases the glycogen content in DF1 cells. Collectively, our results confirm that FOSL2 has a key role in the regulation of the MG content in chicken. This finding is helpful to understand the mechanism of MG metabolism regulation in chicken and provides a new perspective for the production of high-quality broiler and the development of a comprehensive nutritional control strategy.
畜禽动物肌肉中的糖原含量会影响其体内的稳态、生长性能以及屠宰后的肉质。在我们之前的研究中，FOS样2，AP-1转录因子亚基（FOSL2）被确定为与鸡肌肉糖原（MG）含量相关的候选基因，但FOSL2在调节MG含量中的作用仍有待阐明。对来自京星黄鸡高MG含量（HMG）组和低MG含量（LMG）组胸肌组织中的差异表达基因（DEG）进行了差异基因表达分析和加权基因共表达网络分析（WGCNA）。对鉴定出的1171个DEG（LMG与HMG）进行分析，除了……之外，还发现了一些与MG代谢途径相关的其他基因，即……、……、……、……和……。此外，WGCNA显示，在同一共表达模块中，与经典糖原代谢相关的……、……、……、……、……、……、……和其他基因与MG含量相关。另外，除了发现……表达与MG含量呈负相关外，还使用STRING（相互作用基因检索工具）数据库预测了……与……之间可能存在的相互作用。此外，我们研究了慢病毒过表达FOSL2对糖原含量调节的影响，结果表明FOSL2降低了DF1细胞中的糖原含量。总的来说，我们的结果证实FOSL2在调节鸡的MG含量中起关键作用。这一发现有助于理解鸡MG代谢调节的机制，并为优质肉鸡的生产和全面营养控制策略的制定提供了新的视角。
