冷冻保存的人类精液样本可以储存在-80°C吗？

Could cryopreserved human semen samples be stored at -80°C?

作者信息

Vaz Carlos R, Lamim Tamara, Salvador Rafael A, Batschauer Anna P B, Amaral Vera Lucia L, Til David

机构信息

Laboratório de Biotecnologia da Reprodução (LBR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil.

出版信息

JBRA Assist Reprod. 2018 Jun 1;22(2):108-112. doi: 10.5935/1518-0557.20180016.

DOI:10.5935/1518-0557.20180016

PMID:29338138

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5982555/

Abstract

OBJECTIVE

To evaluate storage time effects in cryopreserved human semen samples, kept in the freezer at a controlled temperature of -80°C, on sperm viability after thawing.

METHODS

We used 20 semen samples. Each sample was cryopreserved in 10 fingers, which were divided into five groups: one group was kept in cryogenic canisters throughout the experiment(control), and four groups were kept in a VIP Ultra Low MDF-U76V- PE freezer, with the temperature set at -80°C, for 24, 48, 72 and 96 hours, respectively. After the exposure time, the samples were stored in cryogenic canisters after being thawed. The analyzed parameters were: motility, vitality and mitochondrial activity.

RESULTS

After thawing, we noticed decreased sperm motility, vitality and mitochondrial activity, when comparing the tested groups with the control group, as well as a progressive reduction in the analyzed parameters between the times evaluated.

CONCLUSIONS

Cryopreservation of semen samples at -80°C is potentially harmful to sperm viability, causing damage when submitted to longer exposure times.

摘要

目的

评估保存在-80°C可控温度的冷冻柜中的冷冻人类精液样本的储存时间对解冻后精子活力的影响。

方法

我们使用了20份精液样本。每个样本被冷冻保存在10个小管中，这些小管被分为五组：一组在整个实验过程中保存在低温罐中（对照组），四组分别在温度设定为-80°C的VIP超低温MDF-U76V-PE冷冻柜中保存24、48、72和96小时。暴露时间结束后，样本解冻后保存在低温罐中。分析的参数包括：活力、生存力和线粒体活性。