Brasch Jochen, Freitag-Wolf Sandra, Beck-Jendroschek Vera, Huber Martin
Department of Dermatology, University Hospitals of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Institute of Medical Informatics and Statistics, Christian-Albrechts University of Kiel, Kiel, Germany.
Med Mycol. 2017 Oct 1;55(7):754-762. doi: 10.1093/mmy/myw139.
Treatment of dermatophytoses with currently available antimycotic agents is often tedious and sometimes unsatisfactory. A search for better therapeutic methods-ideally with an immediate fungicidal effect-has, among others, lead to photodynamic procedures as a promising alternative, and recently curcumin was found to be a suitable agent for this application. In this study the effect of photodynamic treatment with curcumin on dermatophytes was tested in vitro. Wells of microtiter plates were filled with conidia of Trichophyton rubrum, Trichophyton interdigitale, Trichophyton terrestre, Microsporum canis, Microsporum gypseum and Epidermophyton floccosum in buffer. Then curcumin was added to the conidia and after 20 min the assays were irradiated one time only with visible light (peak wave length 367 nm, 5 J/cm2). Thereafter the wells were filled up with Sabouraud's glucose broth and in the following fungal growth was measured photometrically. The results showed that all dermatophytes were markedly inhibited depending on the concentration of curcumin. With 5.4 mg/l curcumin plus irradiation fungal growth was significantly suppressed over a period of 96 h (P < .001). Even after 96 h inhibition of T. rubrum was still complete and marked for all other species as well. M. gypseum was least susceptible. Our results are very encouraging to pursue the development of a photodynamic therapy of tinea with curcumin. The outstanding tolerance of curcumin and the innocuousness of the required light are favorable preconditions for this task.
用目前可用的抗真菌剂治疗皮肤癣菌病通常很繁琐，有时效果也不尽人意。寻找更好的治疗方法——理想情况下具有即时杀菌效果——促使人们探索多种方法，光动力疗法成为一种有前景的替代方法，最近发现姜黄素是适用于此的一种药物。在本研究中，对姜黄素光动力疗法治疗皮肤癣菌的效果进行了体外测试。在微量滴定板的孔中加入缓冲液中的红色毛癣菌、指间毛癣菌、土生毛癣菌、犬小孢子菌、石膏样小孢子菌和絮状表皮癣菌的分生孢子。然后将姜黄素加入分生孢子中，20分钟后仅用可见光（峰值波长367nm，5J/cm²）照射一次。此后，向孔中加入沙氏葡萄糖肉汤，随后通过光度法测量真菌生长情况。结果表明，所有皮肤癣菌均受到明显抑制，抑制程度取决于姜黄素的浓度。当姜黄素浓度为5.4mg/l并进行照射时，在96小时内真菌生长受到显著抑制（P<0.001）。即使在96小时后，红色毛癣菌的抑制仍然完全，其他所有菌种的抑制也很明显。石膏样小孢子菌最不易感。我们的结果非常鼓舞人心，促使我们开展用姜黄素进行光动力治疗癣病的研究。姜黄素出色的耐受性以及所需光线的无害性为此项任务提供了有利的前提条件。
