ORCAN——一个用于直系同源基因实时检测和功能注释的基于网络的元服务器。

ORCAN-a web-based meta-server for real-time detection and functional annotation of orthologs.

作者信息

Zielezinski Andrzej, Dziubek Michal, Sliski Jan, Karlowski Wojciech M

出版信息

Bioinformatics. 2017 Apr 15;33(8):1224-1226. doi: 10.1093/bioinformatics/btw825.

DOI:10.1093/bioinformatics/btw825

PMID:28057683

Abstract

SUMMARY

ORCAN (ORtholog sCANner) is a web-based meta-server for one-click evolutionary and functional annotation of protein sequences. The server combines information from the most popular orthology-prediction resources, including four tools and four online databases. Functional annotation utilizes five additional comparisons between the query and identified homologs, including: sequence similarity, protein domain architectures, functional motifs, Gene Ontology term assignments and a list of associated articles. Furthermore, the server uses a plurality-based rating system to evaluate the orthology relationships and to rank the reference proteins by their evolutionary and functional relevance to the query. Using a dataset of ∼1 million true yeast orthologs as a sample reference set, we show that combining multiple orthology-prediction tools in ORCAN increases the sensitivity and precision by 1-2 percent points.

AVAILABILITY AND IMPLEMENTATION

The service is available for free at http://www.combio.pl/orcan/ .

CONTACT

wmk@amu.edu.pl.

SUPPLEMENTARY INFORMATION

Supplementary data are available at Bioinformatics online.

摘要

ORCAN（直系同源物扫描器）是一个基于网络的元服务器，用于对蛋白质序列进行一键式进化和功能注释。该服务器整合了来自最流行的直系同源预测资源的信息，包括四个工具和四个在线数据库。功能注释利用查询序列与已鉴定同源物之间的另外五种比较，包括：序列相似性、蛋白质结构域结构、功能基序、基因本体术语分配以及相关文章列表。此外，该服务器使用基于多元的评分系统来评估直系同源关系，并根据参考蛋白质与查询序列的进化和功能相关性对其进行排名。使用约100万个真酵母直系同源物的数据集作为样本参考集，我们表明在ORCAN中组合多个直系同源预测工具可将灵敏度和精确度提高1至2个百分点。