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蛋白磷酸酶AP2C1对基础抗性和对丁香假单胞菌的防御反应起负调控作用。

Protein phosphatase AP2C1 negatively regulates basal resistance and defense responses to Pseudomonas syringae.

作者信息

Shubchynskyy Volodymyr, Boniecka Justyna, Schweighofer Alois, Simulis Justinas, Kvederaviciute Kotryna, Stumpe Michael, Mauch Felix, Balazadeh Salma, Mueller-Roeber Bernd, Boutrot Freddy, Zipfel Cyril, Meskiene Irute

机构信息

Max F. Perutz Laboratories, University and Medical University of Vienna, Dr Bohrgasse 9, A-1030 Vienna, Austria.

Institute of Biotechnology (IBT), University of Vilnius, Saulėtekio al. 7, LT-10257 Vilnius, Lithuania.

出版信息

J Exp Bot. 2017 Feb 1;68(5):1169-1183. doi: 10.1093/jxb/erw485.

DOI:10.1093/jxb/erw485
PMID:28062592
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5444444/
Abstract

Mitogen-activated protein kinases (MAPKs) mediate plant immune responses to pathogenic bacteria. However, less is known about the cell autonomous negative regulatory mechanism controlling basal plant immunity. We report the biological role of Arabidopsis thaliana MAPK phosphatase AP2C1 as a negative regulator of plant basal resistance and defense responses to Pseudomonas syringae. AP2C2, a closely related MAPK phosphatase, also negatively controls plant resistance. Loss of AP2C1 leads to enhanced pathogen-induced MAPK activities, increased callose deposition in response to pathogen-associated molecular patterns or to P. syringae pv. tomato (Pto) DC3000, and enhanced resistance to bacterial infection with Pto. We also reveal the impact of AP2C1 on the global transcriptional reprogramming of transcription factors during Pto infection. Importantly, ap2c1 plants show salicylic acid-independent transcriptional reprogramming of several defense genes and enhanced ethylene production in response to Pto. This study pinpoints the specificity of MAPK regulation by the different MAPK phosphatases AP2C1 and MKP1, which control the same MAPK substrates, nevertheless leading to different downstream events. We suggest that precise and specific control of defined MAPKs by MAPK phosphatases during plant challenge with pathogenic bacteria can strongly influence plant resistance.

摘要

丝裂原活化蛋白激酶（MAPKs）介导植物对病原菌的免疫反应。然而，关于控制植物基础免疫的细胞自主负调控机制，我们了解得还较少。我们报道了拟南芥MAPK磷酸酶AP2C1作为植物基础抗性和对丁香假单胞菌防御反应的负调控因子的生物学作用。AP2C2是一种与之密切相关的MAPK磷酸酶，也对植物抗性起负调控作用。AP2C1缺失会导致病原菌诱导的MAPK活性增强，在响应病原菌相关分子模式或丁香假单胞菌番茄致病变种（Pto）DC3000时胼胝质沉积增加，以及对Pto细菌感染的抗性增强。我们还揭示了AP2C1对Pto感染期间转录因子全局转录重编程的影响。重要的是，ap2c1植株表现出几种防御基因的水杨酸非依赖性转录重编程以及对Pto响应时乙烯产量增加。这项研究指出了不同的MAPK磷酸酶AP2C1和MKP1对MAPK调控的特异性，它们控制相同的MAPK底物，但导致不同的下游事件。我们认为，在植物受到病原菌攻击期间，MAPK磷酸酶对特定MAPKs进行精确和特异性调控会强烈影响植物抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/17d542aaead0/erw48511.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/e14757e8ba36/erw48501.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/ee05c88603fd/erw48510.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/17d542aaead0/erw48511.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/e14757e8ba36/erw48501.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/a95f85a42ff1/erw48502.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/fa8046228622/erw48503.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/29fcfb7aeee5/erw48504.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/1b852c89be91/erw48505.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/0cff688c1257/erw48506.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/d57c82f9126b/erw48507.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/af838d4eb0bb/erw48508.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/ba51e378e141/erw48509.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/ee05c88603fd/erw48510.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3996/5444444/17d542aaead0/erw48511.jpg

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