Khan Abdul Waheed, Khan Arif-Ullah, Ahmed Touqeer
Department of Pharmacology, Riphah Institute of Pharmaceutical Sciences, Riphah International University Islamabad, Pakistan.
Neurobiology Laboratory, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology Islamabad, Pakistan.
Front Pharmacol. 2016 Dec 21;7:499. doi: 10.3389/fphar.2016.00499. eCollection 2016.
We describe different neuropharmacological effects of crude extract (Vo.Cr). Pentylenetetrazole (PTZ)-induced seizures, elevated plus maze, light-dark box (LDB), open field and thiopental-induced sleeping test models were employed to evaluate Vo.Cr actions in mice. Vo.Cr dose-dependently (100-500 mg/Kg) delayed onset time of myoclonic jerks and tonic-clonic seizures, while decreased duration of tonic-clonic seizures ( < 0.05, < 0.001 vs. saline group). Vo.Cr at 100 and 300-500 mg/Kg doses reduced animals' mortality in PTZ-induced seizures test to 75 and 0%, respectively. Vo.Cr (50-300 mg/Kg) significantly increased time spent and number of entries into open arms, while decreased time spent and number of entries into closed arms ( < 0.05, < 0.01, < 0.001 vs. saline group), measured in elevated plus maze. Vo.Cr (50-300 mg/Kg) increased time spent in light compartment, while decreased time spent in dark compartment ( < 0.01, < 0.001 vs. saline group) in LDB, like caused by diazepam. In open field test, Vo.Cr decreased number of ambulations and rearings frequencies, while increased the number of central squares crossings. In thiopental-induced sleeping test, Vo.Cr (50-300 mg/Kg) decreased onset time of sleep, while increased the duration of sleep ( < 0.05, < 0.01, < 0.001 vs. saline group). These results indicate that possess anticonvulsant, anxiolytic and sedative activities, which provides scientific background for its medicinal application in various neurological ailments, such as epilepsy, anxiety, and insomnia.
我们描述了粗提取物（Vo.Cr）的不同神经药理学作用。采用戊四氮（PTZ）诱导的癫痫发作、高架十字迷宫、明暗箱（LDB）、旷场和硫喷妥钠诱导的睡眠测试模型来评估Vo.Cr对小鼠的作用。Vo.Cr剂量依赖性地（100 - 500毫克/千克）延迟肌阵挛性抽搐和强直阵挛性癫痫发作的发作时间，同时缩短强直阵挛性癫痫发作的持续时间（与生理盐水组相比，P < 0.05，P < 0.001）。在PTZ诱导的癫痫发作试验中，100毫克/千克和300 - 500毫克/千克剂量的Vo.Cr分别将动物死亡率降低至75%和0%。在高架十字迷宫中测量发现，Vo.Cr（50 - 300毫克/千克）显著增加在开放臂停留的时间和进入开放臂的次数，同时减少在封闭臂停留的时间和进入封闭臂的次数（与生理盐水组相比，P < 0.05，P < 0.01，P < 0.001）。在明暗箱中，Vo.Cr（50 - 300毫克/千克）增加在亮区停留的时间，同时减少在暗区停留的时间（与生理盐水组相比，P < 0.01，P < 0.001），类似于地西泮的作用。在旷场试验中，Vo.Cr减少活动次数和直立频率，同时增加穿过中央方格的次数。在硫喷妥钠诱导的睡眠试验中，Vo.Cr（50 - 300毫克/千克）缩短睡眠发作时间，同时增加睡眠时间（与生理盐水组相比，P < 0.05，P < 0.01，P < 0.001）。这些结果表明，Vo.Cr具有抗惊厥、抗焦虑和镇静活性，这为其在各种神经系统疾病如癫痫、焦虑和失眠中的药用应用提供了科学依据。
