Ngo Chung T, Romano-Bertrand Sara, Manguin Sylvie, Jumas-Bilak Estelle
Institut de Recherche pour le Développement France, UMR-MD3, Faculté de PharmacieMontpellier, France; National Institute of Veterinary ResearchHanoi, Vietnam.
UMR 5569 Hydrosciences, Equipe Pathogènes Hydriques, Santé et Environnements, Faculté de Pharmacie, Université de MontpellierMontpellier, France; Département d'Hygiène Hospitalière, Centre Hospitalier Universitaire de MontpellierMontpellier, France.
Front Microbiol. 2016 Dec 23;7:2095. doi: 10.3389/fmicb.2016.02095. eCollection 2016.
The naturally acquired microbiota of can influence vector's susceptibility to and its capacity to transmit them. Microbiota modification is a new challenge to limit disease transmission but it still needs advanced knowledges on bacterial community in , especially in wild and infected specimens from diverse origin and species. Bacterial culture and 16S rRNA gene-PCR associated to Temporal Temperature Gradient Electrophoresis (TTGE) were applied to explore the bacterial diversity in the abdomen of 100 wild specimens (eight species) collected in the Binh Phuoc Province, Vietnam. Culture and PCR-TTGE were complementary. The bacterial richness of the mosquito collection encompassed 105 genera belonging to seven phyla, mostly , , and . , , and in were the most prevalent genera. However, represented by 57 genera was the most diversified phylum in microbiota. The high overall of -associated bacteria is confirmed with, to our knowledge, 51 genera described for the first time in microbiota. However, the diversity per specimen was low with average diversity index and the average Shannon-Wiener score (H) of 4.843 and 5.569, respectively. The most represented bacterial genera were present in <30% of the specimens. Consequently, the core microbiota share by from Binh Phuoc was very narrow, suggesting that microbiota was greatly influenced by local environments. The repertory of bacterial genera in two specimens of and naturally infected by was also described as preliminary results. Finally, this study completed the repertory of bacteria associated to wild . associated-bacteria appeared specimen-dependent rather than mosquitoe species- or group-dependent. Their origin and the existence of -specific bacterial taxa are discussed.
蚊子的自然获得性微生物群可影响其对病原体的易感性及其传播病原体的能力。微生物群改造是限制疾病传播的一项新挑战，但仍需要深入了解蚊子体内的细菌群落，特别是来自不同来源和物种的野生感染标本中的细菌群落。应用细菌培养和与温度梯度凝胶电泳（TTGE）相关的16S rRNA基因PCR技术，探索在越南平阳省采集的100只野生标本（8种蚊子）腹部的细菌多样性。培养和PCR-TTGE是互补的。蚊子样本中的细菌丰富度包括属于7个门的105个属，主要是变形菌门、厚壁菌门和放线菌门。库蚊属、伊蚊属和按蚊属中的不动杆菌属、芽孢杆菌属和微球菌属是最普遍的属。然而，以57个属为代表的变形菌门是蚊子微生物群中最多样化的门。据我们所知，在蚊子微生物群中首次描述了51个属，从而证实了与蚊子相关细菌的总体多样性较高。然而，每个标本的多样性较低，平均多样性指数和平均香农-维纳得分（H）分别为4.843和5.569。大多数代表性细菌属存在于不到30%的标本中。因此，平阳省蚊子的核心微生物群份额非常狭窄，这表明蚊子微生物群受当地环境的影响很大。还初步描述了自然感染疟原虫的两个库蚊属和按蚊属标本中的细菌属谱。最后，本研究完善了与野生蚊子相关的细菌谱。与蚊子相关的细菌似乎依赖于标本，而不是蚊子种类或群体。讨论了它们的来源以及特定于蚊子的细菌类群的存在。
