Diversity of the Bacterial Microbiota of Mosquitoes from Binh Phuoc Province, Vietnam.

The naturally acquired microbiota of can influence vector's susceptibility to and its capacity to transmit them. Microbiota modification is a new challenge to limit disease transmission but it still needs advanced knowledges on bacterial community in , especially in wild and infected specimens from diverse origin and species. Bacterial culture and 16S rRNA gene-PCR associated to Temporal Temperature Gradient Electrophoresis (TTGE) were applied to explore the bacterial diversity in the abdomen of 100 wild specimens (eight species) collected in the Binh Phuoc Province, Vietnam. Culture and PCR-TTGE were complementary. The bacterial richness of the mosquito collection encompassed 105 genera belonging to seven phyla, mostly , , and . , , and in were the most prevalent genera. However, represented by 57 genera was the most diversified phylum in microbiota. The high overall of -associated bacteria is confirmed with, to our knowledge, 51 genera described for the first time in microbiota. However, the diversity per specimen was low with average diversity index and the average Shannon-Wiener score (H) of 4.843 and 5.569, respectively. The most represented bacterial genera were present in <30% of the specimens. Consequently, the core microbiota share by from Binh Phuoc was very narrow, suggesting that microbiota was greatly influenced by local environments. The repertory of bacterial genera in two specimens of and naturally infected by was also described as preliminary results. Finally, this study completed the repertory of bacteria associated to wild . associated-bacteria appeared specimen-dependent rather than mosquitoe species- or group-dependent. Their origin and the existence of -specific bacterial taxa are discussed.