Jiménez Pilar, Cabrero Patricia, Cordoba-Diaz Damian, Cordoba-Diaz Manuel, Garrosa Manuel, Girbés Tomás
Nutrición y Bromatología, Facultad de Medicina, Universidad de Valladolid, Valladolid 47005, Spain.
Farmacia y Tecnología Farmacéutica, Facultad de Farmacia and Instituto Universitario de Farmacia Industrial (IUFI), Universidad Complutense de Madrid, Madrid 28040, Spain.
Molecules. 2017 Jan 6;22(1):95. doi: 10.3390/molecules22010095.
Elderberry contains healthy low molecular weight nutraceuticals and lectins which are sequence-related to the elderberry allergen Sam n1. Some of these lectins are type II ribosome-inactivating proteins. The sensitivity of native lectins present in elderberry fruits and bark to the proteolysis triggered by in vitro simulated gastric and duodenal fluids has been investigated. It was found that these lectins are refractory to proteolysis. Nonetheless, incubation for 5-10 min in a boiling water bath completely sensitized them to the hydrolytic enzymes in vitro. Under these conditions neither total Folin-Ciocalteau's reagent reactive compounds, total anthocyanins and the mixture of cyanidin-3-glucoside plus cyanidin-3-sambubioside, nor antioxidant and free-radical scavenging activities were affected by more than 10% for incubations of up to 20 min. Therefore, short-time heat treatment reduces potential allergy-related risks deriving from elderberry consumption without seriously affecting its properties as an antioxidant and free-radical scavenging food.
接骨木果含有健康的低分子量营养保健品和凝集素，这些凝集素与接骨木果过敏原Sam n1存在序列相关性。其中一些凝集素是II型核糖体失活蛋白。对接骨木果和树皮中存在的天然凝集素在体外模拟胃液和十二指肠液引发的蛋白水解作用下的敏感性进行了研究。发现这些凝集素对蛋白水解具有抗性。尽管如此，在沸水浴中孵育5 - 10分钟可使其在体外对水解酶完全敏感。在这些条件下，对于长达20分钟的孵育，福林 - 酚试剂总反应性化合物、总花青素以及矢车菊素 - 3 - 葡萄糖苷加矢车菊素 - 3 - 接骨木二糖苷的混合物，以及抗氧化和自由基清除活性受影响均不超过10%。因此，短时间热处理可降低食用接骨木果带来的潜在过敏相关风险，同时不会严重影响其作为抗氧化和自由基清除食物的特性。
