Nakayama S, Yamauchi M, Oguchi K
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.
Nihon Yakurigaku Zasshi. 1989 Aug;94(2):137-44. doi: 10.1254/fpj.94.137.
The effect of Laennec (human placenta hydrolysate) on CCl4-induced acute or chronic liver injury in rats was examined. In the acute liver injury induced by CCl4, 0.5 ml/kg for 4 days, intravenous injection of Laennec increased total protein and decreased nonesterified fatty acid in the liver. Subcutaneous injection of Laennec inhibited the decrease of liver phospholipid by CCl4 administration. Both intravenous and subcutaneous injections of Laennec inhibited the increases of serum transaminase (GOT, GPT) levels caused by CCl4. Furthermore, intravenous Laennec inhibited the increase of serum alkaline phosphatase level. Pathological examinations of the liver indicated that both intravenous and subcutaneous injections of Laennec inhibited the loss of cytoplasma and nuclei, vacuolation, swelling and necrosis in the centrizonal hepatocytes caused by CCl4. Intravenous and subcutaneous injection of Laennec also inhibited the increases of GOT and GPT levels in rats with chronic liver injury caused by CCl4, 0.5 ml/kg for 7 weeks. Both intravenous and subcutaneous injections of Laennec minimized the pathological changes of the liver by CCl4 such as vacuolation, necrosis and swelling of nuclei, but did not inhibit the formation of pseudolobules. Thus, no therapeutic difference was noted between intravenous and subcutaneous injections of Laennec.
研究了Laennec（人胎盘水解物）对四氯化碳诱导的大鼠急性或慢性肝损伤的影响。在四氯化碳诱导的急性肝损伤中，静脉注射Laennec，剂量为0.5 ml/kg，连续4天，可增加肝脏总蛋白并降低非酯化脂肪酸。皮下注射Laennec可抑制因给予四氯化碳而导致的肝脏磷脂减少。静脉注射和皮下注射Laennec均能抑制四氯化碳引起的血清转氨酶（谷草转氨酶、谷丙转氨酶）水平升高。此外，静脉注射Laennec可抑制血清碱性磷酸酶水平升高。肝脏病理检查表明，静脉注射和皮下注射Laennec均能抑制四氯化碳引起的肝小叶中央区肝细胞的细胞质和细胞核丢失、空泡化、肿胀和坏死。静脉注射和皮下注射Laennec还能抑制四氯化碳诱导的慢性肝损伤大鼠（剂量为0.5 ml/kg，连续7周）的谷草转氨酶和谷丙转氨酶水平升高。静脉注射和皮下注射Laennec均能使四氯化碳引起的肝脏病理变化（如空泡化、细胞核坏死和肿胀）降至最低，但不抑制假小叶的形成。因此，静脉注射和皮下注射Laennec之间未观察到治疗差异。
