Sakamoto K, Tonooka M, Abe K, Usami K, Kasahara T
Jpn J Pharmacol. 1986 Jul;41(3):424-6. doi: 10.1254/jjp.41.424.
We have investigated the effect of a therapeutic agent for liver disease, Laennec, on the GOT leakage from freshly isolated and primary cultured rat hepatocytes which were treated with CCl4. By treatment with Laennec together with CCl4, the GOT leakage from isolated hepatocytes increased and that from cultured hepatocytes decreased, compared to those incubated only with CCl4. The results suggest that it is better to use primary cultured hepatocytes than to use freshly isolated hepatocytes to evaluate therapeutic agents for liver disease.
我们研究了一种肝病治疗药物Laennec对四氯化碳处理的新鲜分离和原代培养大鼠肝细胞中谷草转氨酶（GOT）泄漏的影响。与仅用四氯化碳孵育的细胞相比，将Laennec与四氯化碳一起处理时，分离肝细胞的GOT泄漏增加，而培养肝细胞的GOT泄漏减少。结果表明，评估肝病治疗药物时，使用原代培养肝细胞比使用新鲜分离的肝细胞更好。
