Han Fei, Ma Guang-Qiang, Yang Ming, Yan Li, Xiong Wei, Shu Ji-Cheng, Zhao Zhi-Dong, Xu Han-Lin
College of Pharmacy, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China.
College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
J Zhejiang Univ Sci B. 2017;18(1):79-84. doi: 10.1631/jzus.B1600377.
This research was undertaken in order to characterize the chemical compositions and evaluate the antioxidant activities of essential oils obtained from different parts of the Origanum vulgare L. It is a medicinal plant used in traditional Chinese medicine for the treatment of heat stroke, fever, vomiting, acute gastroenteritis, and respiratory disorders. The chemical compositions of the three essential oils from different parts of the oregano (leaves-flowers, stems, and roots) were identified by gas chromatography-mass spectrometry (GC-MS). The antioxidant activity of each essential oil was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reducing the power test. Among the essential oils from different parts of the oregano, the leaf-flower oils have the best antioxidant activities, whereas the stem oils are the worst. The results of the DPPH free radical scavenging assay showed that the half maximal inhibitory concentration (IC) values of the essential oils were (0.332±0.040) mg/ml (leaves-flowers), (0.357±0.031) mg/ml (roots), and (0.501±0.029) mg/ml (stems), respectively. Interestingly, the results of reducing the power test also revealed that when the concentration exceeded 1.25 mg/ml, the leaf-flower oils had the highest reducing power; however, the stem oils were the lowest.
本研究旨在表征牛至(Origanum vulgare L.)不同部位所获精油的化学成分,并评估其抗氧化活性。牛至是一种用于治疗中暑、发热、呕吐、急性肠胃炎及呼吸系统疾病的中药材。采用气相色谱 - 质谱联用(GC-MS)法鉴定了牛至不同部位(叶 - 花、茎和根)的三种精油的化学成分。使用2,2 - 二苯基 - 1 - 苦基肼(DPPH)自由基清除试验和还原力测试评估了每种精油的抗氧化活性。在牛至不同部位的精油中,叶 - 花精油的抗氧化活性最佳,而茎精油的抗氧化活性最差。DPPH自由基清除试验结果表明,精油的半数抑制浓度(IC)值分别为(0.332±0.040)mg/ml(叶 - 花)、(0.357±0.031)mg/ml(根)和(0.501±0.029)mg/ml(茎)。有趣的是,还原力测试结果还显示,当浓度超过1.25 mg/ml时,叶 - 花精油的还原力最高;然而,茎精油的还原力最低。