Ghosh B B, Talukder G, Sharma A
Department of Botany, University of Calcutta, India.
Hum Toxicol. 1989 Sep;8(5):349-53. doi: 10.1177/096032718900800503.
Trimethyltin chloride was found to induce cytotoxic damage in vitro in human peripheral blood lymphocytes. Two concentrations (0.5 micrograms and 1.0 micrograms) were added to lymphocytes from male and female subjects in mitogen stimulated and serum supplemented culture medium for 72 h. A dose-related increase of inhibition of replication index (P less than 0.01) and cell division (P less than 0.001) was observed. The frequencies of abnormal cells and chromosomal aberrations such as chromatid and chromosome breaks, dicentrics, triradial and quadriradial configurations were increased significantly (P less than 0.001), as were micronucleus counts (P less than 0.001) and sister chromatid exchanges (P less than 0.001). Endoreduplication, an extremely rare spontaneous event in human lymphocytes, was observed in a few cases at all dose levels. The effects were more pronounced in lymphocytes obtained from habitual smokers.
已发现氯化三甲基锡在体外可诱导人外周血淋巴细胞产生细胞毒性损伤。在有丝分裂原刺激且添加血清的培养基中，将两种浓度（0.5微克和1.0微克）的氯化三甲基锡添加到男性和女性受试者的淋巴细胞中，培养72小时。观察到复制指数抑制（P<0.01）和细胞分裂（P<0.001）呈剂量相关增加。异常细胞的频率以及染色单体和染色体断裂、双着丝粒体、三辐射体和四辐射体构型等染色体畸变显著增加（P<0.001），微核计数（P<0.001）和姐妹染色单体交换（P<0.001）也是如此。在所有剂量水平下，均在少数病例中观察到核内再复制，这在人淋巴细胞中是一种极其罕见的自发事件。这些效应在习惯性吸烟者的淋巴细胞中更为明显。
