Ganguly B B
Biochemistry Unit, Indian Statistical Institute, Calcutta.
Biometals. 1995 Jul;8(3):263-9. doi: 10.1007/BF00143386.
Trimethyltin chloride induced age-related suppression of cell division and cell cycle kinetics in human peripheral blood lymphocytes cultured in RPMI 1640 culture medium supplemented with human AB serum, phytohemagglutinin and bromodeoxyuridine. A high frequency of M1 (first metaphase) cells was seen in cultures treated with a high dose (C1 = 1.0 microgram per culture) and in lymphocytes from donors in the age range 40-70 years. The delay in cell division and cell cycle kinetics may indicate a longer duration in DNA synthesis induced by trimethyltin chloride in aged lymphocytes.
三甲基氯化锡在添加人AB血清、植物血凝素和溴脱氧尿苷的RPMI 1640培养基中培养的人外周血淋巴细胞中，诱导了与年龄相关的细胞分裂抑制和细胞周期动力学变化。在用高剂量（每培养物C1 = 1.0微克）处理的培养物以及年龄在40 - 70岁供体的淋巴细胞中，观察到高频率的M1（第一次中期）细胞。细胞分裂和细胞周期动力学的延迟可能表明三甲基氯化锡在老年淋巴细胞中诱导的DNA合成持续时间更长。
