Robial R, Longatto-Filho A, Roteli-Martins C M, Silveira M F, Stauffert D, Ribeiro G G, Linhares I M, Tacla M, Zonta M A, Baracat E C
Division of Gynecologic Clinic, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Laboratory of Medical Investigation (LIM) 14, Faculty of Medicine, University of São Paulo, São Paulo, Brazil ; Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil ; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal ; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Infect Agent Cancer. 2017 Jan 6;12:3. doi: 10.1186/s13027-016-0111-8. eCollection 2017.
(Ct) is not a disease subject to mandatory reporting in Brazil, and the prevalence rate of this genital infection varies according to the region in which studies are conducted, as well as by the detection technique employed. Ct has been associated with persistence of Human papillomavirus (HPV) infection and the facilitation of cervical carcinoma development. We evaluated the infection and its association with cytology, p16/Ki-67 dual-stained cytology and cervical intraepithelial lesions status in a screening cohort in Brazil.
This was a cross-sectional study of 1481 cervical samples from asymptomatic women aged 18 to 64. Samples were collected for liquid-based cytology and Ct detection by polymerase chain reaction. p16/Ki-67 double staining was performed on samples with abnormal cytology. Statistical analysis was by chi-square and likelihood-ratio tests. Odds ratio (OR) and 95% confidence intervals (95% CI) were determined.
The frequency of Ct was 15.6% and its presence was not associated with detection of p16/Ki-67 [OR = 1.35 (0.5-3.4)]. There was also no association between abnormal cervical cytology and -positivity [OR = 1.21 (0.46-3.2)]. Associations were observed between p16/Ki-67 and high-grade lesions detected by cytology and in biopsies [OR = 3.55 (1.50-8.42) and OR = 19.00 (0.6-7.2), respectively].
The asymptomatic women in our study had a high frequency of Ct infection but this was not associated with p16/Ki-67 detection in samples with abnormal cytology. The expression of p16/Ki-67 was highest in women with high-grade CIN ( = 0.003).
沙眼衣原体（Ct）在巴西并非属于强制报告的疾病，这种生殖器感染的患病率会因开展研究的地区以及所采用的检测技术而有所不同。Ct与人类乳头瘤病毒（HPV）感染的持续存在以及宫颈癌的发生发展有关。我们在巴西的一个筛查队列中评估了Ct感染及其与细胞学、p16/Ki-67双重染色细胞学和宫颈上皮内病变状态的关联。
这是一项对1481例年龄在18至64岁无症状女性宫颈样本的横断面研究。采集样本用于液基细胞学检查以及通过聚合酶链反应检测Ct。对细胞学异常的样本进行p16/Ki-67双重染色。采用卡方检验和似然比检验进行统计分析。确定比值比（OR）和95%置信区间（95%CI）。
Ct的感染率为15.6%，其存在与p16/Ki-67的检测无关[OR = 1.35（0.5 - 3.4）]。宫颈细胞学异常与Ct阳性之间也无关联[OR = 1.21（0.46 - 3.2）]。在细胞学检查和活检中检测到的p16/Ki-67与高级别病变之间存在关联[分别为OR = 3.55（1.50 - 8.42）和OR = 19.00（0.6 - 7.2）]。
我们研究中的无症状女性Ct感染率较高，但这与细胞学异常样本中p16/Ki-67的检测无关。p16/Ki-67的表达在高级别宫颈上皮内瘤变（CIN）女性中最高（P = 0.003）。
