Wissuwa Juliane, Stokke Runar, Fedøy Anita-Elin, Lian Kjersti, Smalås Arne Oskar, Steen Ida Helene
Centre for Geobiology, University of Bergen, N-5020 Bergen, Norway ; Department of Biology, University of Bergen, N-5020 Bergen, Norway.
The Norwegian Structural Biology Center (NorStruct), Department of Chemistry, UIT - The Arctic University of Norway, 9037 Tromsø, Norway.
Stand Genomic Sci. 2016 Feb 24;11:16. doi: 10.1186/s40793-016-0137-y. eCollection 2016.
Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.
嗜热栖热菌属的成员已从全球各种栖息地中分离出来，并且是各种工业应用中靶向酶利用的研究对象。在此，我们报告嗜热淀粉降解嗜热栖热菌菌株12AMOR1的分离及全基因组序列。菌株12AMOR1是从扬马延岛热液喷口处的深海热沉积物中分离得到的。嗜热栖热菌菌株12AMOR1由一条3,410,035 bp的环状染色体和一个32,689 bp的质粒组成，其G + C含量分别为52%和47%。该基因组包含3323个蛋白质编码基因、88种tRNA和10个rRNA操纵子。该分离株能利用一系列糖类、复合多糖和含蛋白质的碳源生长。因此，在基因组中鉴定出了多种编码碳水化合物活性酶（CAZy）和肽酶的基因。糖苷水解酶家族13中一种酶的表达、纯化及特性分析表明，其具有淀粉降解能力和高热稳定性，解链温度为76.4°C。总之，所获得的数据表明这是一种来自海洋热液喷口的具有巨大生物勘探潜力的新分离株。
