Martin R E, Donahue M J
Int J Parasitol. 1989 Sep;19(6):585-96. doi: 10.1016/0020-7519(89)90037-4.
Muscle, hypodermis and gastrointestinal epithelial cells from adult female Ascaris lumbricoides var. suum were found to contain serotonin based upon glyoxylic acid induced histofluorescence and indirect immunolabeling with an antiserotonin monoclonal antibody conjugated to protein A-colloidal gold. Histofluorescence indicated that muscle-hypodermis and intestinal epithelial cells contained significant concentrations of 5-hydroxytryptamine while fluorescence was absent in the nerve cord and cuticle. Immunolabeling at the ultrastructural level indicated that serotonin was sequestered in electron-opaque patches, dense vesicles and mitochondria of the muscle-hypodermis and intestinal tissue. Perfusion of whole worms and isolated tissues with 10(4) M-serotonin further indicated: (1) immunolabeled patches and dense vesicles were often associated with cytoskeletal elements, (2) serotonin did not appear to enter the intestinal or muscle cells by endocytosis, (3) immunolabeled patches examined with energy dispersive X-ray spectrometry (X-ray microanalysis) were found to contain iron at concentrations approximately double that of the surrounding cytoplasm.
基于乙醛酸诱导的组织荧光以及与蛋白A - 胶体金偶联的抗血清素单克隆抗体进行间接免疫标记，发现来自成年雌性猪蛔虫的肌肉、皮下组织和胃肠道上皮细胞含有血清素。组织荧光表明，肌肉 - 皮下组织和肠上皮细胞含有大量浓度的5 - 羟色胺，而神经索和角质层中无荧光。超微结构水平的免疫标记表明，血清素被隔离在肌肉 - 皮下组织和肠组织的电子不透明斑块、致密小泡和线粒体中。用10⁻⁴M血清素灌注整条虫体和分离组织进一步表明：（1）免疫标记的斑块和致密小泡通常与细胞骨架成分相关；（2）血清素似乎不是通过内吞作用进入肠细胞或肌肉细胞；（3）用能量色散X射线光谱法（X射线微分析）检测的免疫标记斑块发现含有铁，其浓度约为周围细胞质的两倍。
