Selvaratnam Chitra, Thevarajoo Suganthi, Goh Kian Mau, Chan Kok-Gan, Chong Chun Shiong
Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5537-5543. doi: 10.1099/ijsem.0.001553.
The genus Roseivirga currently includes five species: Roseivirga ehrenbergii, R. echinicomitans, R. spongicola, R. marina and R. maritima. Marinicola seohaensis SW-152T was renamed as Roseivirgaseohaensis SW-152T and then reclassified again as a later heterotypic synonym of R. ehrenbergii KMM 6017T. In this study, based on average nucleotide identity and digital DNA-DNA hybridization values obtained from in silico methods, together with fatty acid analyses and biochemical tests, we propose to reclassify R. ehrenbergii SW-152 as Roseivirga seohaensis comb. nov. (type strain SW-152T=KCTC 1231T=JCM 12600T). In this work, a Gram-negative, rod-shaped, aerobic and pink-pigmented strain designated as D-25T was isolated from seawater (Desaru Beach, Johor, Malaysia). The 16S rRNA gene analysis revealed that strain D-25T was related to the genus Roseivirga. Strain D-25T was found most closely related to R. seohaensis SW-152T based on average nucleotide identity and digital DNA-DNA hybridization values, phenotypic and chemotaxonomic analyses, indicating that these strains belong to the same species. Thus, it is proposed to split the species R.oseivirga seohaensis into two novel subspecies, Roseivirga seohaensissubsp. seohaensis subsp. nov. (type strain SW-152T=KCTC 12312T=JCM 12600T) and Roseivirga seohaensissubsp. aquiponti subsp. nov. (type strain D-25T=KCTC 42709T=DSM 101709T) and to emend the description of the genus Roseivirga.
埃伦贝格玫瑰海杆菌、棘刺玫瑰海杆菌、海绵玫瑰海杆菌、海洋玫瑰海杆菌和海生玫瑰海杆菌。南海海栖杆菌SW-152T被重新命名为南海玫瑰海杆菌SW-152T，随后又被重新分类为埃伦贝格玫瑰海杆菌KMM 6017T的晚出异名。在本研究中，基于通过计算机方法获得的平均核苷酸同一性和数字DNA-DNA杂交值，以及脂肪酸分析和生化测试，我们建议将埃伦贝格玫瑰海杆菌SW-152重新分类为南海玫瑰海杆菌新组合。（模式菌株SW-152T = KCTC 1231T = JCM 12600T）。在这项工作中，从海水（马来西亚柔佛州迪沙鲁海滩）中分离出一株革兰氏阴性、杆状、需氧且呈粉红色的菌株，命名为D-25T。16S rRNA基因分析表明，菌株D-25T与玫瑰海杆菌属相关。基于平均核苷酸同一性和数字DNA-DNA杂交值、表型和化学分类分析，发现菌株D-25T与南海玫瑰海杆菌SW-152T关系最为密切，表明这些菌株属于同一物种。因此，建议将南海玫瑰海杆菌物种分为两个新亚种，即南海玫瑰海杆菌南海亚种新亚种（模式菌株SW-152T = KCTC 12312T = JCM 12600T）和南海玫瑰海杆菌水桥亚种新亚种（模式菌株D-25T = KCTC 42709T = DSM 101709T），并修订玫瑰海杆菌属的描述。
