和对产毒黄曲霉的抗真菌活性及黄曲霉毒素降解作用
Antifungal Activity and Aflatoxin Degradation of and Against Toxigenic .
作者信息
Ghazvini Roshanak Daie, Kouhsari Ebrahim, Zibafar Ensieh, Hashemi Seyed Jamal, Amini Abolfazl, Niknejad Farhad
机构信息
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Open Microbiol J. 2016 Nov 30;10:197-201. doi: 10.2174/1874285801610010197. eCollection 2016.
Food and feedstuff contamination with aflatoxins (AFTs) is a serious health problem for humans and animals, especially in developing countries. The present study evaluated antifungal activities of two lactic acid bacteria (LAB against growth and aflatoxin production of toxigenic . The mycelial growth inhibition rate of PTCC 5286 was investigated in the presence of PTCC 1644 and PTCC 1744 by the pour plate method. After seven days incubation in yeast extract sucrose broth at 30°C, the mycelial mass was weighed after drying. The inhibitory activity of LAB metabolites against aflatoxin production by was evaluated using HPLC method. and significantly reduced aflatoxin production and growth rate of in comparison with the controls (p≤0.05). LAB reduced total aflatoxins and B, B, G and G fractions by more than 99%. Moreover, LAB metabolites reduced the level of standard AFB, B, G and G from 88.8% to 99.8% (p≤0.05). Based on these findings, and are recommended as suitable biocontrol agents against the growth and aflatoxin production by aflatoxigenic species.
黄曲霉毒素（AFTs）对食品和饲料的污染对人类和动物来说是一个严重的健康问题，尤其是在发展中国家。本研究评估了两种乳酸菌（LAB）对产毒黄曲霉生长和黄曲霉毒素产生的抗真菌活性。通过倾注平板法研究了在PTCC 1644和PTCC 1744存在下PTCC 5286的菌丝体生长抑制率。在30°C的酵母提取物蔗糖肉汤中培养7天后，干燥后称量菌丝体质量。使用HPLC法评估LAB代谢产物对黄曲霉产生黄曲霉毒素的抑制活性。与对照相比，PTCC 1644和PTCC 1744显著降低了黄曲霉毒素的产生和黄曲霉的生长速率（p≤0.05）。LAB使总黄曲霉毒素以及B1、B2、G1和G2组分减少了99%以上。此外，LAB代谢产物使标准AFB1、B2、G1和G2的水平从88.8%降至99.8%（p≤0.05）。基于这些发现，PTCC 1644和PTCC 1744被推荐为对抗产毒黄曲霉菌种生长和黄曲霉毒素产生的合适生物防治剂。
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