Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Department of Traditional Medicine, Faculty of Traditional Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):609-619. doi: 10.22092/ari.2020.351952.1545. eCollection 2021 Summer.
Conventional cancer treatments are costly and have different serious side effects for patients. Natural herbal treatments are widely accepted among people because of their minimal side effects, although there is little scientific knowledge about them. One of these remedies utilizes the root of that has been used for years in Iran to treat different chronic genital diseases. The current study examined the effects of methanolic and ethanolic extracts of (induction of necrosis and apoptosis) on breast cancer (MCF-7), ovarian cancer (A2780), and human cervix cancer (HeLa) cell lines in comparison with normal breast cells. These effects were determined to be morphological alterations in cell light microscopy, by flow cytometry (staining with annexin V and propidium iodide), and by measuring live cells and inhibition concentrations by MTT assay. IC50 of on the MCF-7 cell line (methanolic extract) was 400 µg/ml and for A2780 was 250 µg/ml. The IC50 amount of on the MCF-7 cell line (ethanolic extract) was 750 µg/ml and 1500 for A2780. Results demonstrated that apoptosis and necrosis occurred in MCF-7 and A2780 following the addition of ethanolic and methanolic extracts of to the medium. These findings confirmed the anti-cancer effects of mehthanolic extracts of root and its safety for normal cells; thus, it can be applied in cancer therapy as a novel medication.
传统的癌症治疗方法费用高昂，并且会给患者带来不同程度的严重副作用。由于天然草药治疗的副作用极小，因此在人们中广泛接受，尽管对它们的科学知识了解甚少。其中一种疗法利用了 的根部，多年来伊朗一直将其用于治疗各种慢性生殖器疾病。本研究比较了甲醇和乙醇提取物对乳腺癌（MCF-7）、卵巢癌（A2780）和人宫颈癌（HeLa）细胞系（诱导坏死和凋亡）的影响，以及正常乳腺细胞。这些影响通过细胞光镜下的形态改变、流式细胞术（用 Annexin V 和碘化丙啶染色）以及通过 MTT 测定法测量活细胞和抑制浓度来确定。MCF-7 细胞系（甲醇提取物）中 的 IC50 为 400 µg/ml，A2780 为 250 µg/ml。MCF-7 细胞系（乙醇提取物）中 的 IC50 量为 750 µg/ml，A2780 为 1500 µg/ml。结果表明，向培养基中添加 的甲醇和乙醇提取物后，MCF-7 和 A2780 中发生了凋亡和坏死。这些发现证实了 mehthanolic 提取物对 根的抗癌作用及其对正常细胞的安全性；因此，它可以作为一种新型药物应用于癌症治疗。
