Suter L, Bröcker E B, Ostmeier H, Schumann J, Sorg C
Fachklinik Hornheide, Federal Republic of Germany.
J Cancer Res Clin Oncol. 1989;115(5):459-64. doi: 10.1007/BF00393338.
According to animal experiments, metastasis to a particular organ depends on the phenotype of the tumor cells. Widespread metastatic dissemination including internal organs would therefore, at least in part, depend on the capacity of tumor cells to modulate, resulting in increased phenotypic heterogeneity. We found evidence for this assumption in human melanoma by phenotyping metastases (mainly cutaneous/subcutaneous) from 59 patients by the use of six monoclonal antibodies. Interlesional antigenic heterogeneity was present in 22/33 (67%) patients with disseminated metastases including at least one internal organ, but only in 4/26 (15%) patients whose metastases were restricted to skin and/or skin-draining lymph nodes (P less than or equal to 0.01). Chemotherapy cannot be the main reason for interlesional phenotypic heterogeneity, as seen by comparison of treated and untreated patients. Aneuploid melanoma metastases, as an indication for instability on the chromosomal level, were found in the majority of patients (84%) regardless of their clinical situation. Widespread disease was significantly related to the loss of the cytoplasmatic antigen K-1-2 and to the expression of the 130-kDa membrane antigen A-1-43.
根据动物实验，肿瘤细胞转移至特定器官取决于肿瘤细胞的表型。因此，包括内脏器官在内的广泛转移扩散至少部分取决于肿瘤细胞进行调节的能力，从而导致表型异质性增加。我们通过使用六种单克隆抗体对59例患者的转移灶（主要是皮肤/皮下转移灶）进行表型分析，在人类黑色素瘤中发现了支持这一假设的证据。在22/33（67%）例有包括至少一个内脏器官在内的广泛转移的患者中存在病灶内抗原异质性，但在转移局限于皮肤和/或皮肤引流淋巴结的4/26（15%）例患者中仅1例存在病灶内抗原异质性（P≤0.01）。通过对比接受治疗和未接受治疗的患者发现，化疗并非病灶内表型异质性的主要原因。在大多数患者（84%）中发现了非整倍体黑色素瘤转移灶，这表明在染色体水平存在不稳定性，且与患者的临床情况无关。广泛转移的疾病与细胞质抗原K-1-2的缺失以及130-kDa膜抗原A-1-43的表达显著相关。
