Stenzinger W, Suter L, Schumann J
J Invest Dermatol. 1984 Jun;82(6):569-72. doi: 10.1111/1523-1747.ep12261301.
Nuclei with abnormal (aneuploid) DNA content were detected by flow cytometric analysis in 4 of 39 congenital melanocytic nevi and in 0 of 62 acquired nevi. Three of the 4 nevi with DNA-aneuploidy were greater than 20 cm in their largest diameter. We suggest that DNA aneuploidy is an indicator of a premalignant condition in congenital nevi in cases where histologic examination does not reveal any evidence for malignant melanoma. Cells with abnormal DNA content (aneuploid cells) are not uniformly distributed in giant congenital nevi: in one lesion we observed an area with a high percentage of cells with DNA aneuploidy, areas with a low percentage of aneuploid cells, and parts that were found to be normal by flow cytometric analysis. Nuclear pleomorphism was found histologically in the area with the high percentage of aneuploid cells while the areas with the low percentage of aneuploid cells were histologically normal. Thus, flow cytometry seems to be an additional sensitive method for the detection of nuclear abnormalities that are not always apparent by conventional histology. Increase in the relative amount of growth phase (S-phase cells), indicating elevated proliferative activity, was detected in 6 of 39 congenital pigmented nevi and in 6 of 62 acquired nevi.
通过流式细胞术分析，在39例先天性黑素细胞痣中有4例检测到DNA含量异常（非整倍体），而在62例获得性痣中未检测到。4例DNA非整倍体痣中有3例最大直径大于20 cm。我们认为，在组织学检查未发现任何恶性黑色素瘤证据的情况下，DNA非整倍体是先天性痣恶变前状态的一个指标。DNA含量异常的细胞（非整倍体细胞）在巨大先天性痣中分布并不均匀：在一个病变中，我们观察到一个区域非整倍体细胞百分比很高，一个区域非整倍体细胞百分比很低，还有一些部分通过流式细胞术分析显示为正常。在非整倍体细胞百分比高的区域，组织学上发现核多形性，而非整倍体细胞百分比低的区域组织学上正常。因此，流式细胞术似乎是一种额外的敏感方法，用于检测常规组织学并不总是能明显显示的核异常。在39例先天性色素痣中有6例以及在62例获得性痣中有6例检测到生长阶段（S期细胞）相对量增加，表明增殖活性升高。
