Alharbi Khalid Khalaf, Syed Rabbani, Alharbi Fawiziah Khalaf, Khan Imran Ali
1 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University , Riyadh, Kingdom of Saudi Arabia .
2 Department of Biology Science, College of Science and Arts, Al-Qassim University , Buraidah, Kingdom of Saudi Arabia .
Genet Test Mol Biomarkers. 2017 Jan;21(1):53-57. doi: 10.1089/gtmb.2016.0190.
Obesity is known to be a complex disorder caused by both genetic and environmental factors. Patients with obesity tend to develop cardiovascular disease and type 2 diabetes. Previous studies have revealed that obesity is associated with genetic variations including those found in the apolipoprotein E (APOE) gene, which also affects lipid profiles. Hence, in this study, we aimed to perform a molecular characterization of APOE gene polymorphisms found in overweight subjects within a Saudi population.
A case-control study was performed consisting of 198 cases and 198 controls, selected from participants at the King Saud University. TaqMan genotyping was performed to characterize the APOE gene polymorphisms.
The present study identified the E4 allele of the APOE gene as being significantly associated with obesity in the Saudi population (p = 0.0001). We found a statistically significant difference in the genotype distribution between cases and controls [for E3/E4: OR, 2.16 (95% CI: 1.19-3.91); p = 0.009].
Significant differences were observed in the APOE allele profiles (p < 0.001) and lipid profile parameters, including triglycerides and low-density lipoprotein among the obese patients compared with the non-obese control population.
Our results confirm that APOE variants are associated with obesity in the Saudi population.
众所周知，肥胖是一种由遗传和环境因素共同导致的复杂疾病。肥胖患者易患心血管疾病和2型糖尿病。先前的研究表明，肥胖与包括载脂蛋白E（APOE）基因变异在内的基因变异有关，该基因变异也会影响血脂水平。因此，在本研究中，我们旨在对沙特人群中超重个体的APOE基因多态性进行分子特征分析。
进行了一项病例对照研究，包括从沙特国王大学的参与者中选取的198例病例和198例对照。采用TaqMan基因分型法对APOE基因多态性进行特征分析。
本研究发现，APOE基因的E4等位基因与沙特人群中的肥胖显著相关（p = 0.0001）。我们发现病例组和对照组之间的基因型分布存在统计学显著差异[E3/E4：OR，2.16（95%CI：1.19 - 3.91）；p = 0.009]。
与非肥胖对照组相比，肥胖患者的APOE等位基因谱（p < 0.001）和血脂参数（包括甘油三酯和低密度脂蛋白）存在显著差异。
我们的结果证实，APOE变异与沙特人群中的肥胖有关。
