Department of Nanoengineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.
Department of Nanoengineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA; Department of Polymer Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
J Control Release. 2017 Oct 10;263:185-191. doi: 10.1016/j.jconrel.2017.01.016. Epub 2017 Jan 10.
We reported an erythrocyte membrane-coated nanogel (RBC-nanogel) system with combinatorial antivirulence and responsive antibiotic delivery for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infection. RBC membrane was coated onto the nanogel via a membrane vesicle templated in situ gelation process, whereas the redox-responsiveness was achieved by using a disulfide bond-based crosslinker. We demonstrated that the RBC-nanogels effectively neutralized MRSA-associated toxins in extracellular environment and the toxin neutralization in turn promoted bacterial uptake by macrophages. In intracellular reducing environment, the RBC-nanogels showed an accelerated drug release profile, which resulted in more effective bacterial inhibition. When added to the macrophages infected with intracellular MRSA bacteria, the RBC-nanogels significantly inhibited bacterial growth compared to free antibiotics and non-responsive nanogel counterparts. These results indicate the great potential of the RBC-nanogel system as a new and effective antimicrobial agent against MRSA infection.
我们报道了一种红细胞膜包覆的纳米凝胶（RBC-nanogel）系统，该系统具有组合抗病毒和响应性抗生素递药功能，可用于治疗耐甲氧西林金黄色葡萄球菌（MRSA）感染。RBC 膜通过膜囊泡模板原位凝胶化过程被包覆在纳米凝胶上，而氧化还原响应性则是通过使用二硫键交联剂实现的。我们证明了 RBC-nanogels 可以有效地中和细胞外环境中的 MRSA 相关毒素，而毒素中和作用反过来又促进了巨噬细胞对细菌的摄取。在细胞内还原环境中，RBC-nanogels 表现出加速的药物释放特性，从而更有效地抑制细菌。当添加到感染细胞内 MRSA 细菌的巨噬细胞中时，与游离抗生素和无响应纳米凝胶相比，RBC-nanogels 显著抑制了细菌的生长。这些结果表明，RBC-nanogel 系统作为一种新的、有效的抗 MRSA 感染的抗菌剂具有巨大的潜力。
