Kakuta E, Nomura Y, Morozumi T, Nakagawa T, Nakamura T, Noguchi K, Yoshimura A, Hara Y, Fujise O, Nishimura F, Kono T, Umeda M, Fukuda M, Noguchi T, Yoshinari N, Fukaya C, Sekino S, Numabe Y, Sugano N, Ito K, Kobayashi H, Izumi Y, Takai H, Ogata Y, Takano S, Minabe M, Makino-Oi A, Saito A, Abe Y, Sato S, Suzuki F, Takahashi K, Sugaya T, Kawanami M, Hanada N, Takashiba S, Yoshie H
Department of Oral Microbiology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Japan.
Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, 230-8501, Japan.
BMC Oral Health. 2017 Jan 16;17(1):46. doi: 10.1186/s12903-017-0337-x.
The diagnosis of the progression of periodontitis presently depends on the use of clinical symptoms (such as attachment loss) and radiographic imaging. The aim of the multicenter study described here was to evaluate the diagnostic use of the bacterial content of subgingival plaque recovered from the deepest pockets in assessing disease progression in chronic periodontitis patients.
This study consisted of a 24-month investigation of a total of 163 patients with chronic periodontitis who received trimonthly follow-up care. Subgingival plaque from the deepest pockets was recovered and assessed for bacterial content of Porphyromonas gingivalis, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans using the modified Invader PLUS assay. The corresponding serum IgG titers were measured using ELISA. Changes in clinical parameters were evaluated over the course of 24 months. The sensitivity, specificity, and prediction values were calculated and used to determine cutoff points for prediction of the progression of chronic periodontitis.
Of the 124 individuals who completed the 24-month monitoring phase, 62 exhibited progression of periodontitis, whereas 62 demonstrated stable disease. The P. gingivalis counts of subgingival plaque from the deepest pockets was significantly associated with the progression of periodontitis (p < 0.001, positive predictive value = 0.708).
The P. gingivalis counts of subgingival plaque from the deepest pockets may be associated with the progression of periodontitis.
目前牙周炎进展的诊断依赖于临床症状（如附着丧失）和影像学检查。本文所述多中心研究的目的是评估从最深牙周袋中获取的龈下菌斑细菌含量在评估慢性牙周炎患者疾病进展中的诊断价值。
本研究对163例慢性牙周炎患者进行了为期24个月的调查，每三个月进行一次随访。从最深牙周袋中采集龈下菌斑，采用改良的Invader PLUS分析法评估牙龈卟啉单胞菌、中间普氏菌和伴放线聚集杆菌的细菌含量。采用酶联免疫吸附测定法测量相应的血清IgG滴度。在24个月的过程中评估临床参数的变化。计算敏感性、特异性和预测值，并用于确定慢性牙周炎进展预测的截断点。
在完成24个月监测阶段的124例个体中，62例表现为牙周炎进展，而62例病情稳定。最深牙周袋龈下菌斑中牙龈卟啉单胞菌计数与牙周炎进展显著相关（p < 0.001，阳性预测值 = 0.708）。
最深牙周袋龈下菌斑中牙龈卟啉单胞菌计数可能与牙周炎进展有关。
