Ait Seddik Hamza, Bendali Farida, Cudennec Benoit, Drider Djamel
Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria; Univ. Lille, INRA, Univ. Artois, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France.
Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria.
Res Microbiol. 2017 Apr;168(3):244-254. doi: 10.1016/j.resmic.2016.12.003. Epub 2017 Jan 16.
Sixty-seven (67) lactic acid bacteria (LAB) isolates belonging to Lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. Strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by MALDI-TOF spectrometry and 16S rDNA sequencing as Lactobacillus plantarum (p25lb1 and p98lb1) and Lactobacillus salivarius (p85lb1 and p104lb1). These strains were also able to adhere to human epithelial colorectal adenocarcinoma Caco-2 cells, with percentages ranging from 4.68 to 9.59%. They displayed good survival under conditions mimicking the gastrointestinal environment and remarkably impeded adhesion and invasion of human Caco-2 by Listeria monocytogenes and Enteropathogenic Escherichia coli. It should also be noted that Lb. plantarum p98lb1 was able to reduce in vitro cholesterol concentration by about 32%, offering an additional health attribute.
从人类粪便中分离出67株属于乳酸杆菌属的乳酸菌（LAB），并对其自动聚集和细胞表面疏水性进行测试，以确定它们的黏附能力，这是益生菌筛选的一个先决条件。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和16S核糖体DNA（rDNA）测序，将具有最高自动聚集和细胞表面疏水性得分的菌株鉴定为植物乳杆菌（p25lb1和p98lb1）和唾液乳杆菌（p85lb1和p104lb1）。这些菌株也能够黏附于人上皮结直肠癌Caco-2细胞，黏附百分比在4.68%至9.59%之间。它们在模拟胃肠道环境的条件下表现出良好的存活率，并且显著抑制了单核细胞增生李斯特菌和肠致病性大肠杆菌对人Caco-2细胞的黏附和侵袭。还应注意的是，植物乳杆菌p98lb1能够在体外将胆固醇浓度降低约32%，这提供了一项额外的健康益处。
