Hazra Somoday, Kumar Sourav, Saha Goutam Kumar, Mondal Amal Chandra
Department of Zoology, University of Calcutta, Kolkata, West Bengal, India.
Department of Physiology, Raja Peary Mohan College, Uttarpara, Hooghly, West Bengal, India.
Psychiatry Investig. 2017 Jan;14(1):74-80. doi: 10.4306/pi.2017.14.1.74. Epub 2016 Dec 29.
The aims of the present study were to explore the behavioural effects and to understand the possible mode of action of Bacopa monnieri extract (BME) on chronic unpredictable stress (CUS) induced depressive model and the biochemical alterations such as brain derived neurotrophic factor (BDNF), Akt, cyclic-AMP response element binding (CREB) protein level in the hippocampus of rats.
We examined the effects of chronic administration of BME on CUS exposed rats for 28 days. Behavioural changes were assessed by sucrose consumption and open field test to assess the effect of BME on CUS-induced depression. The mechanisms underlying antidepressant like action of BME was further evaluated by measuring levels of BDNF, Akt, and CREB in the hippocampus of rat brain and compared with the standard tricyclic antidepressant drug imipramine (20 mg/kg body weight).
Exposure to CUS for 28 days produced depression-like behavior in rats, as indicated by significant decreases in sucrose consumption, locomotor activity including decreased BDNF, Akt and CREB levels in the hippocampus. Daily administration of BME at a dose of (80 mg/kg body weight) significantly reverses the behavioral alteration and restored the normal level of BDNF, total and phospho-Akt, total and phospho CREB in the hippocampus of CUS induced rats as compared to vehicle treated control rats.
These findings suggest that BME ameliorates CUS induced behavioural depression in rats and that can be used as a potent therapeutic agent in treating depressive like behavior.
本研究旨在探讨假马齿苋提取物（BME）对慢性不可预测应激（CUS）诱导的抑郁模型大鼠的行为影响，并了解其可能的作用方式，以及对大鼠海马中脑源性神经营养因子（BDNF）、Akt、环磷酸腺苷反应元件结合蛋白（CREB）等生化指标的影响。
我们检测了连续28天对CUS暴露大鼠慢性给予BME的效果。通过蔗糖消耗试验和旷场试验评估行为变化，以评估BME对CUS诱导抑郁的作用。通过测量大鼠脑海马中BDNF、Akt和CREB的水平，并与标准三环类抗抑郁药丙咪嗪（20mg/kg体重）进行比较，进一步评估BME抗抑郁样作用的潜在机制。
暴露于CUS 28天的大鼠出现了抑郁样行为，表现为蔗糖消耗显著减少、自发活动降低，包括海马中BDNF、Akt和CREB水平下降。与溶剂处理的对照大鼠相比，每天给予剂量为（80mg/kg体重）的BME可显著逆转CUS诱导大鼠的行为改变，并恢复海马中BDNF、总Akt和磷酸化Akt、总CREB和磷酸化CREB的正常水平。
这些发现表明，BME可改善CUS诱导的大鼠行为性抑郁，可作为治疗抑郁样行为的有效治疗剂。
