Cao Hieu X, Wang Wenqin, Le Hien T T, Vu Giang T H
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben, 06466 Stadt Seeland, Germany.
School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dong Chuan Road, Shanghai 200240, China.
Int J Genomics. 2016;2016:5078796. doi: 10.1155/2016/5078796. Epub 2016 Dec 20.
Genome editing with engineered nucleases enabling site-directed sequence modifications bears a great potential for advanced plant breeding and crop protection. Remarkably, the RNA-guided endonuclease technology (RGEN) based on the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) is an extremely powerful and easy tool that revolutionizes both basic research and plant breeding. Here, we review the major technical advances and recent applications of the CRISPR-Cas9 system for manipulation of model and crop plant genomes. We also discuss the future prospects of this technology in molecular plant breeding.
利用工程核酸酶进行基因组编辑以实现定点序列修饰，在高等植物育种和作物保护方面具有巨大潜力。值得注意的是，基于成簇规律间隔短回文重复序列（CRISPR）和CRISPR相关蛋白9（Cas9）的RNA引导的核酸内切酶技术（RGEN）是一种极其强大且简便的工具，它彻底改变了基础研究和植物育种。在此，我们综述了CRISPR-Cas9系统在模式植物和作物基因组操作方面的主要技术进展及近期应用。我们还讨论了该技术在分子植物育种中的未来前景。
