Fan Dongsheng, Zhou Shuangyan, Zheng Zhiyuan, Zhu Guo-Yuan, Yao Xiaojun, Yang Ming-Rong, Jiang Zhi-Hong, Bai Li-Ping
State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China.
State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Int J Mol Sci. 2017 Jan 13;18(1):147. doi: 10.3390/ijms18010147.
Eleven new abietane type (‒), and one new kaurane (), diterpenes, together with eleven known compounds (-), were isolated and identified from the stems of , which has been used as a traditional folk Chinese medicine for the treatment of rheumatoid arthritis in China. The structures of new compounds were characterized by means of the interpretation of high-resolution electrospray ionization mass spectrometry (HRESIMS), extensive nuclear magnetic resonance (NMR) spectroscopic data and comparisons of their experimental CD spectra with calculated electronic circular dichroism (ECD) spectra. Compound is the first abietane type diterpene with an lactone ring. Compound was isolated from the plants of the genus for the first time, and compounds - were isolated from for the first time. Triregelin I () showed significant cytotoxicity against A2780 and HepG2 with IC values of 5.88 and 11.74 µM, respectively. It was found that this compound was inactive against MCF-7 cells. The discovery of these twelve new diterpenes not only provided information on chemical substances of , but also contributed to the chemical diversity of natural terpenoids.
从一种在中国被用作治疗类风湿性关节炎的传统民间中药植物的茎中，分离并鉴定出了11种新的枞烷型（-）二萜、1种新的贝壳杉烷（）二萜以及11种已知化合物（-）。借助高分辨率电喷雾电离质谱（HRESIMS）的解析、广泛的核磁共振（NMR）光谱数据以及将其实验性圆二色光谱（CD）与计算得到的电子圆二色光谱（ECD）进行比较，对新化合物的结构进行了表征。化合物是首个具有内酯环的枞烷型二萜。化合物首次从该属植物中分离得到，化合物-则首次从该植物中分离得到。Triregelin I（）对A2780和HepG2显示出显著的细胞毒性，IC值分别为5.88和11.74μM。发现该化合物对MCF-7细胞无活性。这12种新二萜的发现不仅为该植物的化学物质提供了信息，也为天然萜类化合物的化学多样性做出了贡献。
