Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Tianhe District, Guangzhou 510650, China.
Food Chem. 2013 Jan 15;136(2):1110-6. doi: 10.1016/j.foodchem.2012.08.015. Epub 2012 Aug 16.
Seven new (1-7) and three known (8-10) abietane diterpenoids were isolated from the methanolic extract of the aerial parts of Isodon lophanthoides var. graciliflorus (Lamiaceae), a folk Chinese medicine and an herb for functional beverages. They were identified as 16-acetoxylsugiol (1), graciliflorin E (2), graciliflorin F (3), 15-O-methylgraciliflorin F (4), 15-hydroxy-20-deoxocarnosol (5), 3β-hydroxysempervirol (6), 15-hydroxy-1-oxosalvibretol (7), abieta-8,11,13-triene-14,19-diol (8), 6,12,15-trihydroxy-5,8,11,13-abietatetraen-7-one (9), and 3α-hinokiol (10) based on the spectroscopic data including COSY (correlated spectroscopy), HMBC (heteronuclear multiple bond correlation), and HR-ESI-MS (high-resolution electrospray ionization mass spectrometry). All the compounds except 10 were obtained from I. lophanthoides for the first time. Compounds 1, 2, 5, 6, 8, and 9 exhibited in vitro cytotoxicity against A549 (human lung adenocarcinoma), MCF-7 (human breast adenocarcinoma), and HeLa (human cervical carcinoma) cell lines with the IC(50) values of 1.79-52.67 μM.
从中国民间药物及功能型饮料用植物秀丽风轮菜（唇形科）的甲醇提取物中分离得到 7 个新的（1-7）和 3 个已知（8-10）贝壳杉烷二萜类化合物。根据波谱数据（包括 COSY、HMBC 和 HR-ESI-MS），将它们鉴定为 16-乙酰氧基贝壳杉烯醇（1）、秀丽风轮菜苷 E（2）、秀丽风轮菜苷 F（3）、15-O-甲基秀丽风轮菜苷 F（4）、15-羟基-20-去氧卡诺醇（5）、3β-羟基桑皮酮（6）、15-羟基-1-氧代萨尔维托醇（7）、贝壳杉-8,11,13-三烯-14,19-二醇（8）、6,12,15-三羟基-5,8,11,13-贝壳杉四烯-7-酮（9）和 3α-扁柏酚（10）。除化合物 10 外，所有化合物均首次从秀丽风轮菜中分离得到。化合物 1、2、5、6、8 和 9 对 A549（人肺腺癌细胞）、MCF-7（人乳腺癌细胞）和 HeLa（人宫颈癌细胞）细胞系的体外细胞毒性试验表明，它们的 IC50 值为 1.79-52.67 μM。
