Lima Diane A, Cibulski Samuel P, Finkler Fabrine, Teixeira Thais F, Varela Ana Paula M, Cerva Cristine, Loiko Márcia R, Scheffer Camila M, Dos Santos Helton F, Mayer Fabiana Q, Roehe Paulo M
FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Rio Grande do Sul, Brazil.
Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul (UFRGS), Rio Grande do Sul, Brazil.
J Gen Virol. 2017 Apr;98(4):690-703. doi: 10.1099/jgv.0.000711. Epub 2017 Apr 20.
This study is focused on the identification of the faecal virome of healthy chickens raised in high-density, export-driven poultry farms in Brazil. Following high-throughput sequencing, a total of 7743 de novo-assembled contigs were constructed and compared with known nucleotide/amino acid sequences from the GenBank database. Analyses with blastx revealed that 279 contigs (4 %) were related to sequences of eukaryotic viruses. Viral genome sequences (total or partial) indicative of members of recognized viral families, including Adenoviridae, Caliciviridae, Circoviridae, Parvoviridae, Picobirnaviridae, Picornaviridae and Reoviridae, were identified, some of those representing novel genotypes. In addition, a range of circular replication-associated protein encoding DNA viruses were also identified. The characterization of the faecal virome of healthy chickens described here not only provides a description of the viruses encountered in such niche but should also represent a baseline for future studies comparing viral populations in healthy and diseased chicken flocks. Moreover, it may also be relevant for human health, since chickens represent a significant proportion of the animal protein consumed worldwide.
本研究聚焦于巴西高密度、出口导向型家禽养殖场中健康鸡的粪便病毒组鉴定。经过高通量测序，共构建了7743个从头组装的重叠群，并与来自GenBank数据库的已知核苷酸/氨基酸序列进行比较。blastx分析显示，279个重叠群（4%）与真核病毒序列相关。鉴定出了指示公认病毒科成员的病毒基因组序列（全部或部分），包括腺病毒科、杯状病毒科、圆环病毒科、细小病毒科、双节段RNA病毒科、小RNA病毒科和呼肠孤病毒科，其中一些代表新基因型。此外，还鉴定出了一系列编码DNA病毒的环状复制相关蛋白。此处描述的健康鸡粪便病毒组特征不仅提供了在此生态位中遇到的病毒的描述，还应为未来比较健康和患病鸡群中病毒种群的研究提供基线。此外，这可能也与人类健康相关，因为鸡在全球消费的动物蛋白中占很大比例。
