Ishikawa Shigeo, Sugimoto Masahiro, Kitabatake Kenichiro, Tu Micheal, Sugano Ayako, Yamamori Iku, Iba Asuka, Yusa Kazuyuki, Kaneko Miku, Ota Sana, Hiwatari Kana, Enomoto Ayame, Masaru Tomita, Iino Mitsuyoshi
Department of Dentistry, Oral and Maxillofacial Plastic and Reconstructive Surgery, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata, 990-9585, Japan.
Institute for Advanced Biosciences, Keio University, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata, 997-0052, Japan.
Amino Acids. 2017 Apr;49(4):761-770. doi: 10.1007/s00726-017-2378-5. Epub 2017 Jan 18.
The aim of this study is to evaluate the effect of duration after meals for saliva collections for oral cancer detection using metabolomics. Saliva samples were collected from oral cancer patients (n = 22) and controls (n = 44). Saliva from cancer patients was collected 12 h after dinner, and 1.5 and 3.5 h after breakfast. Control subjects fasted >1.5 h prior to saliva collection. Hydrophilic metabolites were analyzed using capillary electrophoresis mass spectrometry. Levels of 51 metabolites differed significantly in controls vs. oral cancer patients at the 12-h fasting time point (P < 0.05). Fifteen and ten metabolites differed significantly at the 1.5- and 3.5-h time points, respectively. The area of under receiver operating characteristic curve for discriminating oral cancer patients from controls was greatest at the 12-h fasting time point. The collection time after meals affects levels of salivary metabolites for oral cancer screening. The 12-h fasting after dinner time point is optimal. This study contributes to design of saliva collection protocols for metabolomics-based biomarker discovery.
本研究旨在评估餐后不同时长采集唾液用于基于代谢组学的口腔癌检测的效果。从口腔癌患者（n = 22）和对照组（n = 44）中采集唾液样本。癌症患者的唾液在晚餐后12小时以及早餐后1.5小时和3.5小时采集。对照受试者在采集唾液前禁食超过1.5小时。使用毛细管电泳质谱法分析亲水性代谢物。在12小时禁食时间点，51种代谢物的水平在对照组和口腔癌患者之间存在显著差异（P < 0.05）。在1.5小时和3.5小时时间点，分别有15种和10种代谢物存在显著差异。用于区分口腔癌患者和对照组的受试者工作特征曲线下面积在12小时禁食时间点最大。餐后采集时间会影响用于口腔癌筛查的唾液代谢物水平。晚餐后12小时禁食时间点是最佳的。本研究有助于设计基于代谢组学的生物标志物发现的唾液采集方案。
