Bakr Ahmed M, El Nahrawy Amany M, Mansour A M, Abou Hammad Ali B
Spectroscopy Department, Physics Research Institute, National Research Centre, 33 El-Bohouth St., Dokki, 12622, Giza, Egypt.
Solid State Physics Department, Physics Research Institute, National Research Centre, 33 El Bohouth St., Dokki, 12622, Giza, Egypt.
Sci Rep. 2025 May 2;15(1):15395. doi: 10.1038/s41598-025-96590-7.
This study aims to develop an innovative drug delivery bio-system using bioglass (BIOGLASS) and biopolymers of Sodium Alginate (SA) and polyvinylpyrrolidone (PVP) in microsphere form as a carrier for Amoxicillin/Clavulanic Acid drug. In this work BIOGLASS/SA-PVP and Amoxicillin/Clavulanic Acid loaded BIOGLASS/SA-PVP microspheres (0%, 5%, 10%, and 15%) were synthesized using the ion crosslinking method technique. The fabricated microspheres were analyzed using FT-IR, FESEM/EDX, and XRD confirming the in-vitro examination. XRD and FTIR data demonstrate the effective creation of the apatite layer and the appearance of new apatite peaks at both 605 cm and 565 cm, distinguishing the prolonged vibrations associated with the [Formula: see text] group. SEM images reveal that the prepared bio-beads have a spherical shape, with sizes falling in the micro-scale. The dielectric constant (ε'), the dielectric loss (ε"), and the AC conductivity (σ) were slow at the frequency range of 4 Hz to 8 MHz at room temperature. The antibacterial examinations of the fabricated microspheres were performed employing agar diffusion procedure against the clinical pathogens Gram and Gram bacteria. The SBF (simulated body fluid) experiments display the formation of a hydroxy appetite coating on the microsphere's surfaces that approves their significant bioactivity. Furthermore, antimicrobial results of BIOGLASS/SA-PVP/Amoxicillin/Clavulanic Acid microspheres reveal a notable impact on the antimicrobial performance. The in-vitro tests established that fabricated bio-microspheres are a promising opportunity for bone tissue engineering (substitutes and regeneration), signifying their promise for bone application.
本研究旨在开发一种创新的药物递送生物系统，该系统使用生物玻璃（BIOGLASS）以及海藻酸钠（SA）和聚乙烯吡咯烷酮（PVP）的生物聚合物制成微球形式，作为阿莫西林/克拉维酸药物的载体。在这项工作中，采用离子交联法技术合成了负载有BIOGLASS/SA - PVP和阿莫西林/克拉维酸的BIOGLASS/SA - PVP微球（0%、5%、10%和15%）。使用傅里叶变换红外光谱（FT - IR）、场发射扫描电子显微镜/能谱仪（FESEM/EDX）和X射线衍射仪（XRD）对制备的微球进行分析，以确认体外检测结果。XRD和FTIR数据表明有效形成了磷灰石层，并且在605 cm和565 cm处出现了新的磷灰石峰，这区分了与[化学式：见原文]基团相关的延长振动。扫描电子显微镜（SEM）图像显示制备的生物微球呈球形，尺寸处于微米级。在室温下，4 Hz至8 MHz频率范围内的介电常数（ε'）、介电损耗（ε"）和交流电导率（σ）变化缓慢。采用琼脂扩散法对制备的微球针对临床病原体革兰氏阳性菌和革兰氏阴性菌进行抗菌检测。模拟体液（SBF）实验表明在微球表面形成了羟基磷灰石涂层，这证明了它们具有显著的生物活性。此外，BIOGLASS/SA - PVP/阿莫西林/克拉维酸微球的抗菌结果显示出对抗菌性能有显著影响。体外测试表明制备的生物微球是骨组织工程（替代和再生）的一个有前景的选择，表明它们在骨应用方面具有潜力。
