Walley Peter G, Hough Gemma, Moore Jonathan D, Carder John, Elliott Marian, Mead Andrew, Jones Julie, Teakle Graham, Barker Guy, Buchanan-Wollaston Vicky, Hand Paul, Pink David, Collier Rosemary
Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB UK.
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY UK.
Mol Breed. 2017;37(1):4. doi: 10.1007/s11032-016-0606-4. Epub 2017 Jan 3.
Domesticated lettuce varieties encompass much morphological variation across a range of crop type groups, with large collections of cultivars and landrace accessions maintained in genebanks. Additional variation not captured during domestication, present in ancestral wild relatives, represents a potentially rich source of alleles that can deliver to sustainable crop production. However, these large collections are difficult and costly to screen for many agronomically important traits. In this paper, we describe the generation of a diversity collection of 96 lettuce and wild species accessions that are amenable to routine phenotypic analysis and their genotypic characterization with a panel of 682 newly developed expressed sequence tag (EST)-linked KASP™ single nucleotide polymorphism (SNP) markers that are anchored to the draft genome assembly. To exemplify the utility of these resources, we screened the collection for putative sources of resistance to currant-lettuce aphid () and carried out association analyses to look for potential SNPs linked to resistance.
驯化的生菜品种在一系列作物类型组中呈现出很大的形态变异，基因库中保存着大量的栽培品种和地方品种种质资源。祖先野生近缘种中存在的、在驯化过程中未被捕获的其他变异，是一个潜在的丰富等位基因来源，可为可持续作物生产提供助力。然而，要筛选这些大量种质资源中的许多重要农艺性状既困难又昂贵。在本文中，我们描述了一个由96份生菜和野生种种质组成的多样性群体的构建，这些种质适合进行常规表型分析，并使用一组682个新开发的、与表达序列标签（EST）连锁的竞争性等位基因特异性PCR（KASP™）单核苷酸多态性（SNP）标记对其进行基因型鉴定，这些标记锚定在基因组草图组装上。为了举例说明这些资源的实用性，我们筛选了该群体中对醋栗叶莴苣蚜（）的假定抗性来源，并进行了关联分析以寻找与抗性相关的潜在SNP。
