Ayelign Abebe, Woldegiorgis Ashagrie Zewdu, Adish Abdulaziz, De Boevre Marthe, Heyndrickx Ellen, De Saeger Sarah
a Department of Bioanalysis, Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences , Ghent University , Ghent , Belgium.
b Center for Food Science and Nutrition, College of Natural Sciences , Addis Ababa University , Addis Ababa , Ethiopia.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 Sep;34(9):1606-1616. doi: 10.1080/19440049.2017.1350290. Epub 2017 Jul 10.
The direct measurement of biomarkers of exposure in biological fluids such as urine has become important for assessing aflatoxin exposure in humans as it is the only tool that integrates exposures from various routes. For this reason, a study was conducted to assess aflatoxin exposure among young children in Ethiopia using urinary biomarkers. A cross-sectional study was conducted in ten Woredas (Districts) from Amhara and Tigray regional states of Ethiopia including 200 children (aged 1-4 years). A total of 200 urine samples were collected from 200 children and assessed for the levels of aflatoxin B (AFB), aflatoxin B (AFB), aflatoxin G (AFG), aflatoxin G (AFG) and aflatoxin M (AFM) using a validated LC-MS/MS method. Aflatoxins were detected in 34/200 (17%) of the urine samples whereby four out of five analysed aflatoxins were detected. AFM was detected in 14/200 (7%) of the urine samples in a range of 0.06-0.07 ng/mL. AFB, AFG and AFG were detected in respectively 9/200 (4.5%), 6/200 (3%) and 5/200 (2.5%) of the urine samples whereas AFB was not detected in any of the samples. In this study, there was no association between the different malnutrition categories (stunted, wasting and underweight) and aflatoxin exposure. However, the biomarker analysis showed a clear exposure of young children to aflatoxins. Therefore, awareness to the public is important to prevent potential health consequences of aflatoxins.
直接测量尿液等生物体液中的暴露生物标志物，对于评估人类黄曲霉毒素暴露情况变得至关重要，因为这是整合各种途径暴露的唯一工具。因此，开展了一项研究，以使用尿液生物标志物评估埃塞俄比亚幼儿的黄曲霉毒素暴露情况。在埃塞俄比亚阿姆哈拉和提格雷地区州的10个沃雷达斯（区）进行了一项横断面研究，包括200名儿童（1 - 4岁）。从200名儿童中总共采集了200份尿液样本，并使用经过验证的液相色谱 - 串联质谱法（LC - MS/MS）评估黄曲霉毒素B（AFB）、黄曲霉毒素B（AFB）、黄曲霉毒素G（AFG）、黄曲霉毒素G（AFG）和黄曲霉毒素M（AFM）的水平。在200份尿液样本中的34份（17%）中检测到了黄曲霉毒素，其中检测到了所分析的五种黄曲霉毒素中的四种。在14/200（7%）的尿液样本中检测到AFM，浓度范围为0.06 - 0.07 ng/mL。AFB、AFG和AFG分别在9/200（4.5%）、6/200（3%）和5/200（2.5%）的尿液样本中被检测到，而在任何样本中均未检测到AFB。在这项研究中，不同的营养不良类别（发育迟缓、消瘦和体重不足）与黄曲霉毒素暴露之间没有关联。然而，生物标志物分析表明幼儿明显暴露于黄曲霉毒素。因此，提高公众意识对于预防黄曲霉毒素可能导致的健康后果很重要。
