Meletiadis J, Curfs-Breuker I, Meis J F, Mouton J W
Clinical Microbiology Laboratory, Attikon University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece
Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands.
Antimicrob Agents Chemother. 2017 Mar 24;61(4). doi: 10.1128/AAC.02372-16. Print 2017 Apr.
The susceptibilities of 1,099 molecularly identified clinical isolates against 8 antifungal drugs were determined using the EUCAST microdilution method. A new simple, objective, and mathematically solid method for determining epidemiological cutoff values (ECOFFs) was developed by derivatizing the MIC distribution and determining the derivatized ECOFF (dECOFF) as the highest MIC with the maximum second derivative. The dECOFFs were similar (95% agreement within 1 dilution) to the EUCAST ECOFFs. Overall, low non-wild-type/resistance rates were found. The highest rates were found for azoles with (2.7 to 9.8%), (7%), and (1.7 to 2.3%) and for echinocandins with (3.3%), (1%), and (1.7%).
采用欧盟CAST微量稀释法测定了1099株经分子鉴定的临床分离株对8种抗真菌药物的敏感性。通过对最小抑菌浓度（MIC）分布进行衍生化处理，并将衍生化的流行病学截断值（dECOFF）确定为具有最大二阶导数的最高MIC，开发了一种新的简单、客观且数学上可靠的确定流行病学截断值（ECOFFs）的方法。dECOFFs与欧盟CAST ECOFFs相似（在1个稀释度范围内95%一致）。总体而言，发现非野生型/耐药率较低。唑类药物中，[具体药物1]的发生率最高（2.7%至9.8%），[具体药物2]为7%，[具体药物3]为1.7%至2.3%；棘白菌素类药物中，[具体药物4]为3.3%，[具体药物5]为1%，[具体药物6]为1.7%。
