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从冰冻淡水池塘分离得到的新种的高质量基因组草图序列。

High quality draft genome sequence of sp. nov., isolated from a frozen freshwater pond.

作者信息

Gong Xianzhe, Skrivergaard Stig, Korsgaard Benjamin Smed, Schreiber Lars, Marshall Ian P G, Finster Kai, Schramm Andreas

机构信息

Section for Microbiology, Department of Bioscience, Aarhus University, Aarhus, Denmark.

Center for Geomicrobiology, Department of Bioscience, Aarhus University, Aarhus, Denmark.

出版信息

Stand Genomic Sci. 2017 Jan 19;12:8. doi: 10.1186/s40793-017-0230-x. eCollection 2017.

Abstract

Strain S3-2, isolated from sediment of a frozen freshwater pond, shares 99% 16S rRNA gene sequence identity with strains of the genus . Strain S3-2 is a facultative anaerobe that lacks the ability to produce violacein but shows antibiotic resistance, psychrotolerance, incomplete denitrification, and fermentation. The draft genome of strain S3-2 has a size of ~5.8 Mbp and contains 5,297 genes, including 115 RNA genes. Based on the phenotypic properties of the strain, the low DNA-DNA hybridization (DDH) values with related genomes (<35%), and the low whole genome-based average nucleotide identity (ANI) (<86%) with other strains within the genus we propose that strain S3-2 is the type strain (= DSM 102223 = LMG 29653) of a new species within this genus. We propose the name sp. nov. to emphasize the capability of the strain to grow at low temperatures.

摘要

从一个冰冻淡水池塘的沉积物中分离出的菌株S3-2,其16S rRNA基因序列与该属的菌株有99%的同一性。菌株S3-2是一种兼性厌氧菌,缺乏产生紫菌素的能力,但具有抗生素抗性、耐冷性、不完全反硝化作用和发酵能力。菌株S3-2的基因组草图大小约为5.8 Mbp,包含5297个基因,其中包括115个RNA基因。基于该菌株的表型特性、与相关基因组的低DNA-DNA杂交(DDH)值(<35%)以及与该属内其他菌株的低全基因组平均核苷酸同一性(ANI)(<86%),我们提出菌株S3-2是该属内一个新物种的模式菌株(=DSM 102223 = LMG 29653)。我们提议将其命名为 sp. nov.,以强调该菌株在低温下生长的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10d5/5244535/d81fe0706447/40793_2017_230_Fig1_HTML.jpg

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