通过高效液相色谱法快速检测耐头孢噻肟的情况。
The rapid detection of cefotaxime-resistant by HPLC.
作者信息
Robinson Andrew M, Medlicott Natalie J, Ussher James E
机构信息
Department of Microbiology & Immunology, University of Otago, Dunedin, New Zealand; Department of Microbiology & Immunology, University of Otago, Dunedin, New Zealand.
School of Pharmacy, University of Otago, Dunedin, New Zealand; School of Pharmacy, University of Otago, Dunedin, New Zealand.
出版信息
Future Sci OA. 2016 Sep 9;2(4):FSO142. doi: 10.4155/fsoa-2016-0042. eCollection 2016 Dec.
AIM
Antibiotic resistance mediated by extended-spectrum β-lactamases (ESBL) and AmpC β-lactamases is widespread and increasingly common, often rendering empiric antibiotic therapy ineffective. In septicemia, delays in initiating effective antibiotic therapy are associated with worse clinical outcomes. With current phenotypic antimicrobial susceptibility testing methods, there is often a delay of 18-24 h before the susceptibility of an isolate is known.
RESULTS
Using an HPLC assay, breakdown of the third-generation cephalosporin cefotaxime by ESBL- and AmpC- β-lactamase-producing organisms could be detected within 90 min with 86.4% sensitivity and 100% specificity; sensitivity for ESBL detection was 100%.
CONCLUSION
This assay could be readily established in any clinical laboratory with an HPLC to rapidly detect ESBL-producing .
目的
由超广谱β-内酰胺酶（ESBL）和AmpCβ-内酰胺酶介导的抗生素耐药性广泛存在且日益常见，常常导致经验性抗生素治疗无效。在败血症中，延迟启动有效的抗生素治疗与更差的临床结局相关。使用当前的表型抗菌药物敏感性检测方法，通常在分离株的药敏结果已知之前会有18至24小时的延迟。
结果
使用高效液相色谱（HPLC）检测法，在90分钟内即可检测到产ESBL和AmpCβ-内酰胺酶的微生物对第三代头孢菌素头孢噻肟的分解，灵敏度为86.4%，特异性为100%；检测ESBL的灵敏度为100%。
结论
在任何配备HPLC的临床实验室中都可轻松建立此检测法，以快速检测产ESBL的情况。
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