Rathnayake Nilminie, Gieselmann Dirk-Rolf, Heikkinen Anna Maria, Tervahartiala Taina, Sorsa Timo
Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, 17177 Stockholm, Sweden.
Division of Periodontology, Dentognostics GmbH, 07745 Jena, Germany.
Diagnostics (Basel). 2017 Jan 20;7(1):7. doi: 10.3390/diagnostics7010007.
Human saliva is an easily accessible biological fluid and contains a variety of disease-related biomarkers, which makes it a potential diagnostic medium. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires the development of non-invasive screening and diagnostic technologies, and is among the main goals of oral fluid researchers. The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth-rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) is demanding. Several factors influence their expression and release; these factors include the intracellular location, the molecular size and the flow characteristics of the biological fluid. The type of saliva/oral fluid utilized for the diagnostics affects the analysis. High sensitivity together with sophisticated methods and techniques are essential to get a useful outcome. We describe here a recently developed mouth-rinse that is practical, convenient and inexpensive, as well as PISF chair-side/point of care (PoC) lateral-flow active matrix metalloproteinase (aMMP-8) immunoassays to detect, predict and monitor the course and treatment of periodontitis and peri-implantitis.
人类唾液是一种易于获取的生物流体，含有多种与疾病相关的生物标志物，这使其成为一种潜在的诊断介质。使用唾液/口腔液生物标志物来识别口腔和全身状况的临床应用需要开发非侵入性筛查和诊断技术，这是口腔液研究人员的主要目标之一。分析唾液和口腔液（即漱口水、龈沟液（GCF）和种植体周围炎龈沟液（PISF））中特定疾病的口腔和全身生物标志物具有挑战性。有几个因素会影响它们的表达和释放；这些因素包括细胞内位置、分子大小和生物流体的流动特性。用于诊断的唾液/口腔液类型会影响分析。高灵敏度以及精密的方法和技术对于获得有用的结果至关重要。我们在此描述一种最近开发的漱口水，它实用、方便且价格低廉，以及用于检测、预测和监测牙周炎和种植体周围炎病程及治疗的PISF床旁/即时检测（PoC）侧向流动活性基质金属蛋白酶（aMMP-8）免疫测定法。
