一种用于高分辨率纵向成像斑马鱼幼体尾巴和后躯干的四孔培养皿。
A Four-Well Dish for High-Resolution Longitudinal Imaging of the Tail and Posterior Trunk of Larval Zebrafish.
作者信息
Weijts Bart, Tkachenko Eugene, Traver David, Groisman Alex
机构信息
1 Department of Cellular and Molecular Medicine, UC San Diego , La Jolla, California.
2 School of Medicine, UC San Diego , La Jolla, California.
出版信息
Zebrafish. 2017 Oct;14(5):489-491. doi: 10.1089/zeb.2016.1406. Epub 2017 Jan 24.
Abstract
We describe the design, fabrication, and applications of a four-well dish for imaging of the trunk of larval zebrafish. The dish facilitates immobilization of anesthetized zebrafish larvae, with their tails gently pushed against a microscope cover glass, enabling longitudinal imaging at 24-72 h postfertilization using high-resolution objective lenses.
摘要
我们描述了一种用于幼虫斑马鱼躯干成像的四孔培养皿的设计、制造和应用。该培养皿有助于固定麻醉后的斑马鱼幼虫，其尾巴轻轻抵靠在显微镜盖玻片上，从而能够在受精后24至72小时使用高分辨率物镜进行纵向成像。
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