通过疏水编码在水中对寡肽进行序列选择性结合。
Sequence-Selective Binding of Oligopeptides in Water through Hydrophobic Coding.
机构信息
Department of Chemistry, Iowa State University , Ames, Iowa 50011-3111, United States.
出版信息
J Am Chem Soc. 2017 Feb 15;139(6):2188-2191. doi: 10.1021/jacs.6b12949. Epub 2017 Jan 31.
Abstract
A general method for sequence-specific binding of peptides remains elusive despite decades of research. By creating an array of "hydrophobically coded dimples" on the surface of surface-core doubly cross-linked micelles, we synthesized water-soluble nanoparticle receptors to recognize peptides by the location, number, and nature of their hydrophobic side chains. Minute differences in the side chains could be distinguished, and affinities up to 20 nM were obtained for biologically active oligopeptides in water.
摘要
尽管已经进行了数十年的研究，但仍然难以找到一种通用的方法来实现肽的序列特异性结合。通过在表面-核双重交联胶束的表面上创建一系列“疏水编码的凹陷”，我们合成了水溶性纳米颗粒受体，通过疏水侧链的位置、数量和性质来识别肽。可以区分侧链的微小差异，并且在水中对于生物活性寡肽可以获得高达 20 nM 的亲和力。
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