Zealy Richard W, Wrenn Samuel P, Davila Sylvia, Min Kyung-Won, Yoon Je-Hyun
Department of Biochemistry and Molecular Biology, College of Medicine, Medical University of South Carolina, Charleston, SC, USA.
Wiley Interdiscip Rev RNA. 2017 Sep;8(5). doi: 10.1002/wrna.1414. Epub 2017 Jan 28.
microRNA (miRNA) and RNA-binding proteins (RBPs) have been studied widely in post-transcriptional gene regulation. Previous work has focused on defining how miRNA and RBPs modulate target mRNA decay and translation as well as investigating how they interplay each other. Emerging studies indicate that certain RBPs other than the AGO-family proteins directly interact with mature miRNAs. These findings implicate competitive binding of RBPs to target miRNAs, sequestration of miRNAs from AGO, promotion of AGO binding to miRNAs, and transfer of miRNAs from RBPs to AGO. Recent work also indicates that AGO-free cytoplasmic miRNAs establish complexes with novel miRNA-binding proteins (miRBPs). This review covers the recent discovery of novel miRBPs, offering a new perspective on the miRNA-mediated gene silencing mechanism. WIREs RNA 2017, 8:e1414. doi: 10.1002/wrna.1414 For further resources related to this article, please visit the WIREs website.
微小RNA（miRNA）和RNA结合蛋白（RBP）在转录后基因调控方面已得到广泛研究。以往的工作主要集中在确定miRNA和RBP如何调节靶mRNA的降解和翻译，以及研究它们之间如何相互作用。新出现的研究表明，除AGO家族蛋白外，某些RBP可直接与成熟miRNA相互作用。这些发现提示RBP与靶miRNA的竞争性结合、miRNA与AGO的隔离、AGO与miRNA结合的促进以及miRNA从RBP向AGO的转移。近期研究还表明，不含AGO的细胞质miRNA可与新型miRNA结合蛋白（miRBP）形成复合物。本综述涵盖了新型miRBP的最新发现，为miRNA介导的基因沉默机制提供了新的视角。WIREs RNA 2017, 8:e1414. doi: 10.1002/wrna.1414 有关本文的更多资源，请访问WIREs网站。
